Team:Brown-Stanford/Lab/Protocols

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<li><a href="/Team:Brown-Stanford/Team">Team</a></li>
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<li><a href="/Team:Brown-Stanford/Lab/Notebook">Notebook</a></li>
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<li id="active"><a href="#" id="current">Protocols</a></li>
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{{:Team:Brown-Stanford/Templates/Content}}
== '''Protocols''' ==
== '''Protocols''' ==
=== '''Media''' ===
=== '''Media''' ===
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*[[Team:Brown-Stanford/Lab/Protocols/BG11|BG-11]]
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*[[Team:Brown-Stanford/Lab/Protocols/BG11|'''M1''' - BG-11]]
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*[[Team:Brown-Stanford/Lab/Protocols/LB|LB (Lysogeny broth)]]
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*[[Team:Brown-Stanford/Lab/Protocols/LB|'''M2''' - LB (Lysogeny broth)]]
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*[[Team:Brown-Stanford/Lab/Protocols/ATCC1376|ATCC medium: 1376 (''Bacillus pasteurii'' NH4-YE medium)]]
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*[[Team:Brown-Stanford/Lab/Protocols/ATCC1376|'''M3''' - ATCC medium: 1376 (''Bacillus pasteurii'' NH4-YE medium)]]
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*[[Team:Brown-Stanford/Lab/Protocols/Enrichment|Enrichment media]]
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*[[Team:Brown-Stanford/Lab/Protocols/Enrichment|'''M4''' - Enrichment media]]
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*[[Team:Brown-Stanford/Lab/Protocols/Nutrient|Nutrient agar with urea]]
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*[[Team:Brown-Stanford/Lab/Protocols/Nutrient|'''M5''' - Nutrient agar with urea]]
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*[[Team:Brown-Stanford/Lab/Protocols/SOB|SOB (Super Optimal Broth) and SOC (Super Optimal broth with Catabolite repression)]]
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*[[Team:Brown-Stanford/Lab/Protocols/SOB|'''M6''' - SOB (Super Optimal Broth) and SOC (Super Optimal Broth with Catabolite repression)]]
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*[[Team:Brown-Stanford/Lab/Protocols/Bang|'''M7''' - Bang Media]]
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*[[Team:Brown-Stanford/Lab/Protocols/UreaseTestPlates|'''M8''' - Urease Test Plates]]
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=== '''Competency and Transformations''' ===
 
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*[http://partsregistry.org/Help:Transformation_Protocol Registry Distribution Transformation]
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==== '''S. pasteurii Transformation Media''' ====
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*[https://2008.igem.org/Team:Hawaii/Protocols/Competent_Cells TOP10 Competency Protocol]
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*[[Team:Brown-Stanford/Lab/Protocols/2xSMM|'''M9''' - 2xSMM]]
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*[[Team:Brown-Stanford/Lab/Protocols/FastCaCl|Fast CaCl<sub>2</sub> Combined Competency and Transformation]]
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*[[Team:Brown-Stanford/Lab/Protocols/smmp|'''M10''' - SMMP ]]
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*[[Team:Brown-Stanford/Lab/Protocols/4xPenassay|'''M11''' - Penassay]]
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*[[Team:Brown-Stanford/Lab/Protocols/TE buffer|'''M12''' - TE buffer]]
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*[[Team:Brown-Stanford/Lab/Protocols/PEG solution|'''M13''' - PEG solution]]
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*[[Team:Brown-Stanford/Lab/Protocols/DM3 plates|'''M14''' - DM3 plates]]
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=== '''Equipment''' ===
 
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=== '''Biobrick Constructions''' ===
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*[[Team:Brown-Stanford/Lab/Protocols/PhusionPCR|'''BC2''' - Phusion® PCR]]
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*[[Team:Brown-Stanford/Lab/Protocol/BiobrickRestrictionDigest|'''BC3''' - Biobrick Restriction Digest]]
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*[[Team:Brown-Stanford/Lab/Protocol/T4Ligation|'''BC4''' - T4 Ligation]]
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=== '''Competency and Transformations''' ===
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*[[Team:Brown-Stanford/Lab/Protocols/RegistryTransformation|'''CT1''' - Registry Distribution Transformation]]
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*[[Team:Brown-Stanford/Lab/Protocols/RegistryCompetency|'''CT2''' - Registry Distribution Competency]]
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*[[Team:Brown-Stanford/Lab/Protocols/CaCl|'''CT3''' - CaCl<sub>2</sub> Competency]]
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*[[Team:Brown-Stanford/Lab/Protocols/FastCaCl|'''CT4''' - Fast CaCl<sub>2</sub> Combined Competency and Transformation]]
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*[[Team:Brown-Stanford/Lab/Protocols/ProtoplastTransform|'''CT5''' - Protoplast Transformation]]
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*[[Team:Brown-Stanford/Lab/Protocols/Anabaena|'''CT6''' - Conjugal transfer into Anabaena]]
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*[[Team:Brown-Stanford/Lab/Protocols/NEB 5-alpha|'''CT7''' - NEB 5-alpha High-Efficiency Transformation]]
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=== '''Assembly''' ===
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*[[Team:Brown-Stanford/Lab/Protocols/Gibson|'''AY1''' - Gibson Assembly]]
{{:Team:Brown-Stanford/Templates/Foot}}
{{:Team:Brown-Stanford/Templates/Foot}}
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=== '''Assays''' ===
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*[[Team:Brown-Stanford/Lab/Protocols/Conductivity|'''AS1''' - Ureolysis and Conductivity]]
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=== '''Construction, Transformation, Balloon Flights, Misc''' ===
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*[[Team:Brown-Stanford/Lab/Protocols/BalloonFlight|'''BF1''' - Balloon Flight]]
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*[[Team:Brown-Stanford/Lab/Protocols/CementationColumn|'''CM1''' - Cementation Column]]

Latest revision as of 18:58, 28 September 2011

Brown-Stanford
iGEM
Back to
iGEM HQ

Protocols

Media


S. pasteurii Transformation Media


Biobrick Constructions

Competency and Transformations

Assembly

Assays

Construction, Transformation, Balloon Flights, Misc

Retrieved from "http://2011.igem.org/Team:Brown-Stanford/Lab/Protocols"