"
Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
(Difference between revisions)
(→Assembly) |
(→Assays) |
||
| (7 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
<ul id="subHeaderList"> | <ul id="subHeaderList"> | ||
<li><a href="/Team:Brown-Stanford/Team">Team</a></li> | <li><a href="/Team:Brown-Stanford/Team">Team</a></li> | ||
| + | <li><a href="/Team:Brown-Stanford/Lab/Parts">Parts</a></li> | ||
<li><a href="/Team:Brown-Stanford/Lab/Notebook">Notebook</a></li> | <li><a href="/Team:Brown-Stanford/Lab/Notebook">Notebook</a></li> | ||
<li id="active"><a href="#" id="current">Protocols</a></li> | <li id="active"><a href="#" id="current">Protocols</a></li> | ||
| Line 31: | Line 32: | ||
*[[Team:Brown-Stanford/Lab/Protocols/PEG solution|'''M13''' - PEG solution]] | *[[Team:Brown-Stanford/Lab/Protocols/PEG solution|'''M13''' - PEG solution]] | ||
*[[Team:Brown-Stanford/Lab/Protocols/DM3 plates|'''M14''' - DM3 plates]] | *[[Team:Brown-Stanford/Lab/Protocols/DM3 plates|'''M14''' - DM3 plates]] | ||
| + | |||
| + | |||
| + | === '''Biobrick Constructions''' === | ||
| + | *[[Team:Brown-Stanford/Lab/Protocols/PhusionPCR|'''BC2''' - Phusion® PCR]] | ||
| + | *[[Team:Brown-Stanford/Lab/Protocol/BiobrickRestrictionDigest|'''BC3''' - Biobrick Restriction Digest]] | ||
| + | *[[Team:Brown-Stanford/Lab/Protocol/T4Ligation|'''BC4''' - T4 Ligation]] | ||
=== '''Competency and Transformations''' === | === '''Competency and Transformations''' === | ||
| Line 45: | Line 52: | ||
*[[Team:Brown-Stanford/Lab/Protocols/Gibson|'''AY1''' - Gibson Assembly]] | *[[Team:Brown-Stanford/Lab/Protocols/Gibson|'''AY1''' - Gibson Assembly]] | ||
| - | |||
| - | |||
{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} | ||
| + | |||
| + | === '''Assays''' === | ||
| + | *[[Team:Brown-Stanford/Lab/Protocols/Conductivity|'''AS1''' - Ureolysis and Conductivity]] | ||
=== '''Construction, Transformation, Balloon Flights, Misc''' === | === '''Construction, Transformation, Balloon Flights, Misc''' === | ||
Latest revision as of 18:58, 28 September 2011
-
REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
-
Lab
-
Partners
-
Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
Protocols
Media
- M1 - BG-11
- M2 - LB (Lysogeny broth)
- M3 - ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- M4 - Enrichment media
- M5 - Nutrient agar with urea
- M6 - SOB (Super Optimal Broth) and SOC (Super Optimal Broth with Catabolite repression)
- M7 - Bang Media
- M8 - Urease Test Plates
S. pasteurii Transformation Media
Biobrick Constructions
Competency and Transformations
- CT1 - Registry Distribution Transformation
- CT2 - Registry Distribution Competency
- CT3 - CaCl2 Competency
- CT4 - Fast CaCl2 Combined Competency and Transformation
- CT5 - Protoplast Transformation
- CT6 - Conjugal transfer into Anabaena
- CT7 - NEB 5-alpha High-Efficiency Transformation
Assembly
