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Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
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REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
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Lab
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Partners
-
Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
Protocols
Media
- BG-11
- LB (Lysogeny broth)
- ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- Enrichment media
- Nutrient agar with urea
- SOB (Super Optimal Broth) and SOC (Super Optimal broth with Catabolite repression)
Competency and Transformations
- [http://partsregistry.org/Help:Transformation_Protocol Registry Distribution Transformation]
- TOP10 Competency Protocol
- CaCl2 Competency
- Fast CaCl2 Combined Competency and Transformation
Assembly
Equipment
