Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
Protocols
Media
- BG-11
- LB (Lysogeny broth)
- ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- Enrichment media
- Nutrient agar with urea
- SOB (Super Optimal Broth) and SOC (Super Optimal broth with Catabolite repression)
Competency and Transformations
- Registry Distribution Transformation
- TOP10 Competency Protocol
- CaCl2 Competency
- Fast CaCl2 Combined Competency and Transformation
Assembly
Equipment