Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
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*[[Team:Brown-Stanford/Lab/Protocols/CaCl|'''CT3''' - CaCl<sub>2</sub> Competency]] | *[[Team:Brown-Stanford/Lab/Protocols/CaCl|'''CT3''' - CaCl<sub>2</sub> Competency]] | ||
*[[Team:Brown-Stanford/Lab/Protocols/FastCaCl|'''CT4''' - Fast CaCl<sub>2</sub> Combined Competency and Transformation]] | *[[Team:Brown-Stanford/Lab/Protocols/FastCaCl|'''CT4''' - Fast CaCl<sub>2</sub> Combined Competency and Transformation]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/ProtoplastTransform|'''CT5''' - Protoplast Transformation]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/Anabaena|'''CT6''' - Conjugal transfer into Anabaena]] | ||
=== '''Assembly''' === | === '''Assembly''' === |
Revision as of 00:16, 18 August 2011
Protocols
Media
- M1 - BG-11
- M2 - LB (Lysogeny broth)
- M3 - ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- M4 - Enrichment media
- M5 - Nutrient agar with urea
- M6 - SOB (Super Optimal Broth) and SOC (Super Optimal Broth with Catabolite repression)
- M7 - Bang Media
Competency and Transformations
- CT1 - Registry Distribution Transformation
- CT2 - Registry Distribution Competency
- CT3 - CaCl2 Competency
- CT4 - Fast CaCl2 Combined Competency and Transformation
- CT5 - Protoplast Transformation
- CT6 - Conjugal transfer into Anabaena
Assembly
Equipment