Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
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=== '''Competency and Transformations''' === | === '''Competency and Transformations''' === | ||
- | *[ | + | *[[Team:Brown-Stanford/Lab/Protocols/RegistryTranformation|Registry Distribution Transformation]] |
*[https://2008.igem.org/Team:Hawaii/Protocols/Competent_Cells TOP10 Competency Protocol] | *[https://2008.igem.org/Team:Hawaii/Protocols/Competent_Cells TOP10 Competency Protocol] | ||
*[[Team:Brown-Stanford/Lab/Protocols/CaCl|CaCl<sub>2</sub> Competency]] | *[[Team:Brown-Stanford/Lab/Protocols/CaCl|CaCl<sub>2</sub> Competency]] |
Revision as of 18:31, 5 July 2011
Protocols
Media
- BG-11
- LB (Lysogeny broth)
- ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- Enrichment media
- Nutrient agar with urea
- SOB (Super Optimal Broth) and SOC (Super Optimal broth with Catabolite repression)
Competency and Transformations
- Registry Distribution Transformation
- TOP10 Competency Protocol
- CaCl2 Competency
- Fast CaCl2 Combined Competency and Transformation
Assembly
Equipment