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Team:UEA-JIC Norwich/polyatailing
From 2011.igem.org
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6. Incubate at 72˚C for 20 minutes.<br> | 6. Incubate at 72˚C for 20 minutes.<br> | ||
7. Proceed to cloning. | 7. Proceed to cloning. | ||
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| + | This is linked to the pGEM-T easy protocol. | ||
Latest revision as of 21:41, 21 September 2011
UNIVERSITY OF EAST ANGLIA-JOHN INNES CENTRE
1. Add 1.5µl purified PCR blunt-ended DNA fragments to a PCR tube.
2. Add 10mM dATP at a final concentration of 0.2Mm to the tube.
3. Add 0.6µl of 25mM MgCl2 at a final concentration of 1.5mM to the mix.
4. Add 1µl of TAQ master mix to the mix.
5. Add dH2O to make a final concentration of 10µl.
6. Incubate at 72˚C for 20 minutes.
7. Proceed to cloning.
This is linked to the pGEM-T easy protocol.
