Team:UEA-JIC Norwich/Methods

From 2011.igem.org

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High Efficiency Transformation Protocol
High Efficiency Transformation Protocol
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Culture Preparation Protocol
Culture Preparation Protocol
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[[File:Happy_scientist.gif|Culture Preparation Protocol|https://2011.igem.org/Team:UEA-JIC_Norwich/cultureprep]]
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[[File:Happy_scientist.gif|Culture Preparation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/cultureprep]]
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Glycerol Stock Solution Protocol  
Glycerol Stock Solution Protocol  
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[[File:Happy_scientist.gif|Glycerol Stock Solution Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/glycerolstock]]
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[[File:Happy_scientist.gif|Glycerol Stock Solution Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/glycerolstock]]
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Qiagen Miniprep Protocol
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[[File:Happy_scientist.gif|Qiagen Miniprep Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/miniprep]]
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Gel Electrophoresis Protocol
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/miniprep"> Qiagen Miniprep Protocol </a>
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[[File:Happy_scientist.gif|Gel Electrophoresis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/electrophoresis]]
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PCR Protocol
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/electrophoresis"> Gel Electrophoresis Protocol </a>
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[[File:Happy_scientist.gif|PCR Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pcr]]
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PEG Transformation Protocol
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/pcr"> PCR protocol </a>
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[[File:Happy_scientist.gif|PEG Transformation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pegtransformation]]
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Algae Transformation using glass beads
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/pegtransformation"> PEG Transformation protocol </a>
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[[File:Happy_scientist.gif|Algae Transformation using glass beads Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeglassbeads]]
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Algae Transformation using electroporation
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/algaeglassbeads"> Algae Transformation using glass beads protocol </a>
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[[File:Happy_scientist.gif|Algae Transformation using electroporation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeelectroporation]]
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Site Directed Mutagenesis Protocol
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/algaeelectroporation"> Algae Transformation using electroporation protocol </a>
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[[File:Happy_scientist.gif|Site Directed Mutagenesis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/sdm]]
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pGEM T-easy Vector Cloning protocol
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/sdm"> Site Directed Mutagenesis Protocol </a>
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[[File:Happy_scientist.gif|pGEM T-easy Vector Cloning Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/vectorcloning]]
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Restriction Digest protocol
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/vectorcloning"> pGEM T-easy Vector Cloning Protocol </a>
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[[File:Happy_scientist.gif|Restriction Digest Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/rdigest]]
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Poly "A" Tailing Protocol
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/rdigest"> Restriction Digest Protocol </a>
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[[File:Happy_scientist.gif|Poly "A" Tailing PCR Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/polyatailing]]
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Ligation Protocol
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/polyatailing"> Poly "A" tailing Protocol </a>
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[[File:Happy_scientist.gif|Ligation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/ligation]]
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Gel Extraction Protocol
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○<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/ligation"> Ligation Protocol </a>
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[[File:Happy_scientist.gif|Gel Extraction Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/gelextract]]

Latest revision as of 21:18, 21 September 2011

University of East Anglia-JIC

UNIVERSITY OF EAST ANGLIA-JOHN INNES CENTRE

Research methodsbanner.jpg



Click on the happy scientist to open the protocol


High Efficiency Transformation Protocol
High Efficiency Transformation Protocol
Culture Preparation Protocol
Culture Preparation Protocol
Glycerol Stock Solution Protocol
Glycerol Stock Solution Protocol
Qiagen Miniprep Protocol
Qiagen Miniprep Protocol
Gel Electrophoresis Protocol
Gel Electrophoresis Protocol
PCR Protocol
PCR Protocol
PEG Transformation Protocol
PEG Transformation Protocol
Algae Transformation using glass beads
Algae Transformation using glass beads Protocol
Algae Transformation using electroporation
Algae Transformation using electroporation Protocol
Site Directed Mutagenesis Protocol
Site Directed Mutagenesis Protocol
pGEM T-easy Vector Cloning protocol
pGEM T-easy Vector Cloning Protocol
Restriction Digest protocol
Restriction Digest Protocol
Poly "A" Tailing Protocol
Poly "A" Tailing PCR Protocol
Ligation Protocol
Ligation Protocol
Gel Extraction Protocol

Gel Extraction Protocol