Team:UEA-JIC Norwich/Methods

From 2011.igem.org

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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/transformation"> High Efficiency Transformation Protocol </a>
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/cultureprep"> Culture Preparation Protocol </a>
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<h1>Click on the happy scientist to open the protocol
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High Efficiency Transformation Protocol
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[[File:Happy_scientist.gif|High Efficiency Transformation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/transformation]]
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/glycerolstock"> Glycerol Stock Solution Protocol </a>
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Culture Preparation Protocol
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[[File:Happy_scientist.gif|Culture Preparation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/cultureprep]]
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/miniprep"> Qiagen Miniprep Protocol </a>
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Glycerol Stock Solution Protocol  
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[[File:Happy_scientist.gif|Glycerol Stock Solution Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/glycerolstock]]
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/electrophoresis"> Gel Electrophoresis Protocol </a>
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Qiagen Miniprep Protocol
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[[File:Happy_scientist.gif|Qiagen Miniprep Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/miniprep]]
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/pcr"> PCR protocol </a>
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Gel Electrophoresis Protocol
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[[File:Happy_scientist.gif|Gel Electrophoresis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/electrophoresis]]
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/pegtransformation"> PEG Transformation protocol </a>
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PCR Protocol
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[[File:Happy_scientist.gif|PCR Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pcr]]
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/algaeglassbeads"> Algae Transformation using glass beads protocol </a>
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PEG Transformation Protocol
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[[File:Happy_scientist.gif|PEG Transformation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pegtransformation]]
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/algaeelectroporation"> Algae Transformation using electroporation protocol </a>
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Algae Transformation using glass beads
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[[File:Happy_scientist.gif|Algae Transformation using glass beads Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeglassbeads]]
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<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/starchprep"> Starch Preparation protocol </a>
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Algae Transformation using electroporation
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<p style="color:#000000">1. Wash starch powder with 10mL distilled water and resuspend.
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<p style="color:#000000">2. Centrifuge for five minutes at 4000rpm.
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[[File:Happy_scientist.gif|Algae Transformation using electroporation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeelectroporation]]
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<p style="color:#000000">3. Pour off supernatant.
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<p style="color:#000000">4. Resuspend with 500µL Ethanol.
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Site Directed Mutagenesis Protocol
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<p style="color:#000000">5. Centrifuge for five minutes at 4000rpm.
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<p style="color:#000000">6. Resuspend in 500µL TAP.
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[[File:Happy_scientist.gif|Site Directed Mutagenesis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/sdm]]
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<p style="color:#000000">7. Centrifuge for five minutes at 4000rpm, pour off supernatant, and repeat once.
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<p style="color:#000000">8. Resuspend in 500µL TAP with sucrose.
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pGEM T-easy Vector Cloning protocol
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[[File:Happy_scientist.gif|pGEM T-easy Vector Cloning Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/vectorcloning]]
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Restriction Digest protocol
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[[File:Happy_scientist.gif|Restriction Digest Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/rdigest]]
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Poly "A" Tailing Protocol
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[[File:Happy_scientist.gif|Poly "A" Tailing PCR Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/polyatailing]]
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Ligation Protocol
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[[File:Happy_scientist.gif|Ligation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/ligation]]
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Gel Extraction Protocol
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[[File:Happy_scientist.gif|Gel Extraction Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/gelextract]]

Latest revision as of 21:18, 21 September 2011

University of East Anglia-JIC

UNIVERSITY OF EAST ANGLIA-JOHN INNES CENTRE

Research methodsbanner.jpg



Click on the happy scientist to open the protocol


High Efficiency Transformation Protocol
High Efficiency Transformation Protocol
Culture Preparation Protocol
Culture Preparation Protocol
Glycerol Stock Solution Protocol
Glycerol Stock Solution Protocol
Qiagen Miniprep Protocol
Qiagen Miniprep Protocol
Gel Electrophoresis Protocol
Gel Electrophoresis Protocol
PCR Protocol
PCR Protocol
PEG Transformation Protocol
PEG Transformation Protocol
Algae Transformation using glass beads
Algae Transformation using glass beads Protocol
Algae Transformation using electroporation
Algae Transformation using electroporation Protocol
Site Directed Mutagenesis Protocol
Site Directed Mutagenesis Protocol
pGEM T-easy Vector Cloning protocol
pGEM T-easy Vector Cloning Protocol
Restriction Digest protocol
Restriction Digest Protocol
Poly "A" Tailing Protocol
Poly "A" Tailing PCR Protocol
Ligation Protocol
Ligation Protocol
Gel Extraction Protocol

Gel Extraction Protocol

Retrieved from "http://2011.igem.org/Team:UEA-JIC_Norwich/Methods"