Team:UEA-JIC Norwich/Methods

From 2011.igem.org

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Latest revision as of 21:18, 21 September 2011

University of East Anglia-JIC

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 {{Banner}}
+[[file:Research_methodsbanner.jpg|center]]
-<html>
-<head>
-</head>
-<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/transformation"> High Efficiency Transformation Protocol </a>
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 <br>
-<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/cultureprep"> Culture Preparation Protocol </a>
+<p style="color:#000000">
+<center>
+<h1>Click on the happy scientist to open the protocol
+</h1>
+<Br>
+High Efficiency Transformation Protocol
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+[[File:Happy_scientist.gif|High Efficiency Transformation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/transformation]]
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-<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/glycerolstock"> Glycerol Stock Solution Protocol </a>
+Culture Preparation Protocol
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+[[File:Happy_scientist.gif|Culture Preparation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/cultureprep]]
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-<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/miniprep"> Qiagen Miniprep Protocol </a>
+Glycerol Stock Solution Protocol
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+[[File:Happy_scientist.gif|Glycerol Stock Solution Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/glycerolstock]]
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-<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/electrophoresis"> Gel Electrophoresis Protocol </a>
+Qiagen Miniprep Protocol
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+[[File:Happy_scientist.gif|Qiagen Miniprep Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/miniprep]]
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-<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/pcr"> PCR protocol </a>
+Gel Electrophoresis Protocol
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+[[File:Happy_scientist.gif|Gel Electrophoresis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/electrophoresis]]
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-<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/pegtransformation"> PEG Transformation protocol </a>
+PCR Protocol
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+[[File:Happy_scientist.gif|PCR Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pcr]]
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-<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/algaeglassbeads"> Algae Transformation using glass beads protocol </a>
+PEG Transformation Protocol
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+[[File:Happy_scientist.gif|PEG Transformation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pegtransformation]]
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-<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/algaeelectroporation"> Algae Transformation using electroporation protocol </a>
+Algae Transformation using glass beads
-<p style="color:#000000">1. Transfer 50ml of algae culture into four 50ml screw top tubes.
+<br>
-<p style="color:#000000">2. Place all four tubes in the eppendorf centrifuge at 800rpm for 9 minutes at 20-22°C.
+[[File:Happy_scientist.gif|Algae Transformation using glass beads Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeglassbeads]]
-<p style="color:#000000">3. Immediately pour contents of tubes into glass beaker and keep tubes upside down so no unwanted suspension mixes with the pellet.
+<br>
-<p style="color:#000000">4. For all tubes resuspend cells in 250µl of TAP and sucrose media.
+Algae Transformation using electroporation
-<p style="color:#000000">5. Add linearised G-Luc plasmid (5µL)to the suspension and place on ice.
+<br>
-<p style="color:#000000">6. Transfer 250µL of algae suspension into 1mL cuvette.
+[[File:Happy_scientist.gif|Algae Transformation using electroporation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeelectroporation]]
-<p style="color:#000000">7. Subject cuvettes to electroporation at 2200v/ cm and place back on ice.
+<br>
-<p style="color:#000000">8. Add 500µL of starch solution to each cuvette and aspirate gently.
+Site Directed Mutagenesis Protocol
-<p style="color:#000000">9. Add 750µL of each sample onto an LB plate.
+<br>
-<p style="color:#000000">10.Parafilm the lid and plate to seal and place in a light incubator.
+[[File:Happy_scientist.gif|Site Directed Mutagenesis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/sdm]]
+<br>
+pGEM T-easy Vector Cloning protocol
+<br>
-<h1 style="font-family:verdana;color:green">Starch Preparation</h1>
+[[File:Happy_scientist.gif|pGEM T-easy Vector Cloning Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/vectorcloning]]
-<p style="color:#000000">1. Wash starch powder with 10mL distilled water and resuspend.
+<br>
-<p style="color:#000000">2. Centrifuge for five minutes at 4000rpm.
+Restriction Digest protocol
-<p style="color:#000000">3. Pour off supernatant.
+<br>
-<p style="color:#000000">4. Resuspend with 500µL Ethanol.
+[[File:Happy_scientist.gif|Restriction Digest Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/rdigest]]
-<p style="color:#000000">5. Centrifuge for five minutes at 4000rpm.
+<br>
-<p style="color:#000000">6. Resuspend in 500µL TAP.
+Poly "A" Tailing Protocol
-<p style="color:#000000">7. Centrifuge for five minutes at 4000rpm, pour off supernatant, and repeat once.
+<br>
-<p style="color:#000000">8. Resuspend in 500µL TAP with sucrose.
+[[File:Happy_scientist.gif|Poly "A" Tailing PCR Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/polyatailing]]
+<br>
+Ligation Protocol
-</html>
+<br>
+[[File:Happy_scientist.gif|Ligation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/ligation]]
+<br>
+Gel Extraction Protocol
+<br>
+[[File:Happy_scientist.gif|Gel Extraction Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/gelextract]]