Team:Brown-Stanford/Lab/Protocols

From 2011.igem.org

(Difference between revisions)
(→Protocols)
(→Assays)
 
(52 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Brown-Stanford/Templates/Main}}
{{:Team:Brown-Stanford/Templates/Main}}
 +
<html>
 +
<div id="subHeader">
 +
<ul id="subHeaderList">
 +
<li><a href="/Team:Brown-Stanford/Team">Team</a></li>
 +
<li><a href="/Team:Brown-Stanford/Lab/Parts">Parts</a></li>
 +
<li><a href="/Team:Brown-Stanford/Lab/Notebook">Notebook</a></li>
 +
<li id="active"><a href="#" id="current">Protocols</a></li>
 +
<li><a href="/Team:Brown-Stanford/Lab/Safety">Safety</a></li>
 +
</ul>
 +
</div>
 +
</html>
 +
{{:Team:Brown-Stanford/Templates/Content}}
== '''Protocols''' ==
== '''Protocols''' ==
-
== Media ==
+
=== '''Media''' ===
-
*<a href="/Team:Brown-Stanford/Lab/Protocols/BG11">BG-11 (Blue-green 11)</a>
+
*[[Team:Brown-Stanford/Lab/Protocols/BG11|'''M1''' - BG-11]]
-
*<a href="/Team:Brown-Stanford/Lab/Protocols/LB">LB (Lysogeny broth)</a>
+
*[[Team:Brown-Stanford/Lab/Protocols/LB|'''M2''' - LB (Lysogeny broth)]]
-
*<a href="/Team:Brown-Stanford/Lab/Protocols/ATCC1376">ATCC Medium 1376 (S. pasteurii culture media)</a>
+
*[[Team:Brown-Stanford/Lab/Protocols/ATCC1376|'''M3''' - ATCC medium: 1376 (''Bacillus pasteurii'' NH4-YE medium)]]
-
*Nutrient agar
+
*[[Team:Brown-Stanford/Lab/Protocols/Enrichment|'''M4''' - Enrichment media]]
-
*Enrichment media
+
*[[Team:Brown-Stanford/Lab/Protocols/Nutrient|'''M5''' - Nutrient agar with urea]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/SOB|'''M6''' - SOB (Super Optimal Broth) and SOC (Super Optimal Broth with Catabolite repression)]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/Bang|'''M7''' - Bang Media]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/UreaseTestPlates|'''M8''' - Urease Test Plates]]
 +
 +
==== '''S. pasteurii Transformation Media''' ====
 +
*[[Team:Brown-Stanford/Lab/Protocols/2xSMM|'''M9''' - 2xSMM]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/smmp|'''M10''' - SMMP ]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/4xPenassay|'''M11''' - Penassay]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/TE buffer|'''M12''' - TE buffer]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/PEG solution|'''M13''' - PEG solution]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/DM3 plates|'''M14''' - DM3 plates]]
 +
 +
 +
=== '''Biobrick Constructions''' ===
 +
*[[Team:Brown-Stanford/Lab/Protocols/PhusionPCR|'''BC2''' - Phusion® PCR]]
 +
*[[Team:Brown-Stanford/Lab/Protocol/BiobrickRestrictionDigest|'''BC3''' - Biobrick Restriction Digest]]
 +
*[[Team:Brown-Stanford/Lab/Protocol/T4Ligation|'''BC4''' - T4 Ligation]]
 +
 +
=== '''Competency and Transformations''' ===
 +
 +
*[[Team:Brown-Stanford/Lab/Protocols/RegistryTransformation|'''CT1''' - Registry Distribution Transformation]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/RegistryCompetency|'''CT2''' - Registry Distribution Competency]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/CaCl|'''CT3''' - CaCl<sub>2</sub> Competency]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/FastCaCl|'''CT4''' - Fast CaCl<sub>2</sub> Combined Competency and Transformation]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/ProtoplastTransform|'''CT5''' - Protoplast Transformation]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/Anabaena|'''CT6''' - Conjugal transfer into Anabaena]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/NEB 5-alpha|'''CT7''' - NEB 5-alpha High-Efficiency Transformation]]
 +
 +
=== '''Assembly''' ===
 +
 +
*[[Team:Brown-Stanford/Lab/Protocols/Gibson|'''AY1''' - Gibson Assembly]]
{{:Team:Brown-Stanford/Templates/Foot}}
{{:Team:Brown-Stanford/Templates/Foot}}
 +
 +
=== '''Assays''' ===
 +
*[[Team:Brown-Stanford/Lab/Protocols/Conductivity|'''AS1''' - Ureolysis and Conductivity]]
 +
 +
=== '''Construction, Transformation, Balloon Flights, Misc''' ===
 +
 +
 +
*[[Team:Brown-Stanford/Lab/Protocols/BalloonFlight|'''BF1''' - Balloon Flight]]
 +
*[[Team:Brown-Stanford/Lab/Protocols/CementationColumn|'''CM1''' - Cementation Column]]

Latest revision as of 18:58, 28 September 2011

Brown-Stanford
iGEM
Back to
iGEM HQ

Protocols

Media


S. pasteurii Transformation Media


Biobrick Constructions

Competency and Transformations

Assembly

Assays

Construction, Transformation, Balloon Flights, Misc

Retrieved from "http://2011.igem.org/Team:Brown-Stanford/Lab/Protocols"