Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
(Difference between revisions)
(→Protocols) |
(→Assays) |
||
(3 intermediate revisions not shown) | |||
Line 55: | Line 55: | ||
=== '''Assays''' === | === '''Assays''' === | ||
- | + | *[[Team:Brown-Stanford/Lab/Protocols/Conductivity|'''AS1''' - Ureolysis and Conductivity]] | |
=== '''Construction, Transformation, Balloon Flights, Misc''' === | === '''Construction, Transformation, Balloon Flights, Misc''' === |
Latest revision as of 18:58, 28 September 2011
Protocols
Media
- M1 - BG-11
- M2 - LB (Lysogeny broth)
- M3 - ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- M4 - Enrichment media
- M5 - Nutrient agar with urea
- M6 - SOB (Super Optimal Broth) and SOC (Super Optimal Broth with Catabolite repression)
- M7 - Bang Media
- M8 - Urease Test Plates
S. pasteurii Transformation Media
Biobrick Constructions
Competency and Transformations
- CT1 - Registry Distribution Transformation
- CT2 - Registry Distribution Competency
- CT3 - CaCl2 Competency
- CT4 - Fast CaCl2 Combined Competency and Transformation
- CT5 - Protoplast Transformation
- CT6 - Conjugal transfer into Anabaena
- CT7 - NEB 5-alpha High-Efficiency Transformation
Assembly