Team:Brown-Stanford/Lab/Protocols
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{{:Team:Brown-Stanford/Templates/Main}} | {{:Team:Brown-Stanford/Templates/Main}} | ||
+ | <html> | ||
+ | <div id="subHeader"> | ||
+ | <ul id="subHeaderList"> | ||
+ | <li><a href="/Team:Brown-Stanford/Team">Team</a></li> | ||
+ | <li><a href="/Team:Brown-Stanford/Lab/Parts">Parts</a></li> | ||
+ | <li><a href="/Team:Brown-Stanford/Lab/Notebook">Notebook</a></li> | ||
+ | <li id="active"><a href="#" id="current">Protocols</a></li> | ||
+ | <li><a href="/Team:Brown-Stanford/Lab/Safety">Safety</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </html> | ||
+ | {{:Team:Brown-Stanford/Templates/Content}} | ||
== '''Protocols''' == | == '''Protocols''' == | ||
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*[[Team:Brown-Stanford/Lab/Protocols/UreaseTestPlates|'''M8''' - Urease Test Plates]] | *[[Team:Brown-Stanford/Lab/Protocols/UreaseTestPlates|'''M8''' - Urease Test Plates]] | ||
+ | |||
+ | ==== '''S. pasteurii Transformation Media''' ==== | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/2xSMM|'''M9''' - 2xSMM]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/smmp|'''M10''' - SMMP ]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/4xPenassay|'''M11''' - Penassay]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/TE buffer|'''M12''' - TE buffer]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/PEG solution|'''M13''' - PEG solution]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/DM3 plates|'''M14''' - DM3 plates]] | ||
+ | |||
+ | |||
+ | === '''Biobrick Constructions''' === | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/PhusionPCR|'''BC2''' - Phusion® PCR]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocol/BiobrickRestrictionDigest|'''BC3''' - Biobrick Restriction Digest]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocol/T4Ligation|'''BC4''' - T4 Ligation]] | ||
=== '''Competency and Transformations''' === | === '''Competency and Transformations''' === | ||
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*[[Team:Brown-Stanford/Lab/Protocols/ProtoplastTransform|'''CT5''' - Protoplast Transformation]] | *[[Team:Brown-Stanford/Lab/Protocols/ProtoplastTransform|'''CT5''' - Protoplast Transformation]] | ||
*[[Team:Brown-Stanford/Lab/Protocols/Anabaena|'''CT6''' - Conjugal transfer into Anabaena]] | *[[Team:Brown-Stanford/Lab/Protocols/Anabaena|'''CT6''' - Conjugal transfer into Anabaena]] | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/NEB 5-alpha|'''CT7''' - NEB 5-alpha High-Efficiency Transformation]] | ||
=== '''Assembly''' === | === '''Assembly''' === | ||
*[[Team:Brown-Stanford/Lab/Protocols/Gibson|'''AY1''' - Gibson Assembly]] | *[[Team:Brown-Stanford/Lab/Protocols/Gibson|'''AY1''' - Gibson Assembly]] | ||
+ | {{:Team:Brown-Stanford/Templates/Foot}} | ||
+ | === '''Assays''' === | ||
+ | *[[Team:Brown-Stanford/Lab/Protocols/Conductivity|'''AS1''' - Ureolysis and Conductivity]] | ||
+ | |||
+ | === '''Construction, Transformation, Balloon Flights, Misc''' === | ||
- | |||
- | + | *[[Team:Brown-Stanford/Lab/Protocols/BalloonFlight|'''BF1''' - Balloon Flight]] | |
*[[Team:Brown-Stanford/Lab/Protocols/CementationColumn|'''CM1''' - Cementation Column]] | *[[Team:Brown-Stanford/Lab/Protocols/CementationColumn|'''CM1''' - Cementation Column]] |
Latest revision as of 18:58, 28 September 2011
Protocols
Media
- M1 - BG-11
- M2 - LB (Lysogeny broth)
- M3 - ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- M4 - Enrichment media
- M5 - Nutrient agar with urea
- M6 - SOB (Super Optimal Broth) and SOC (Super Optimal Broth with Catabolite repression)
- M7 - Bang Media
- M8 - Urease Test Plates
S. pasteurii Transformation Media
Biobrick Constructions
Competency and Transformations
- CT1 - Registry Distribution Transformation
- CT2 - Registry Distribution Competency
- CT3 - CaCl2 Competency
- CT4 - Fast CaCl2 Combined Competency and Transformation
- CT5 - Protoplast Transformation
- CT6 - Conjugal transfer into Anabaena
- CT7 - NEB 5-alpha High-Efficiency Transformation
Assembly