Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
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==== '''S. pasteurii Transformation Media''' ==== | ==== '''S. pasteurii Transformation Media''' ==== | ||
- | *[[Team:Brown-Stanford/Lab/Protocols/ | + | *[[Team:Brown-Stanford/Lab/Protocols/2xSMM|'''M8''' - 2xSMM]] |
- | *[[Team:Brown-Stanford/Lab/Protocols/SMMP|''' | + | *[[Team:Brown-Stanford/Lab/Protocols/SMMP|'''M9''' - SMMP ]] |
*[[Team:Brown-Stanford/Lab/Protocols/UreaseTestPlates|'''M8''' - Urease Test Plates]] | *[[Team:Brown-Stanford/Lab/Protocols/UreaseTestPlates|'''M8''' - Urease Test Plates]] | ||
*[[Team:Brown-Stanford/Lab/Protocols/UreaseTestPlates|'''M8''' - Urease Test Plates]] | *[[Team:Brown-Stanford/Lab/Protocols/UreaseTestPlates|'''M8''' - Urease Test Plates]] |
Revision as of 06:49, 27 September 2011
Protocols
Media
- M1 - BG-11
- M2 - LB (Lysogeny broth)
- M3 - ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- M4 - Enrichment media
- M5 - Nutrient agar with urea
- M6 - SOB (Super Optimal Broth) and SOC (Super Optimal Broth with Catabolite repression)
- M7 - Bang Media
- M8 - Urease Test Plates
S. pasteurii Transformation Media
- M8 - 2xSMM
- M9 - SMMP
- M8 - Urease Test Plates
- M8 - Urease Test Plates
- M8 - Urease Test Plates
- M8 - Urease Test Plates
Competency and Transformations
- CT1 - Registry Distribution Transformation
- CT2 - Registry Distribution Competency
- CT3 - CaCl2 Competency
- CT4 - Fast CaCl2 Combined Competency and Transformation
- CT5 - Protoplast Transformation
- CT6 - Conjugal transfer into Anabaena
Assembly