Team:Cambridge/Experiments/Reflectin Thin Films VII

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Looking at this we can see that by breathing we have been able to shift the colour of the film from blue to yellow. The presence of one dominant peak is an indication of the thickness of the film as we are only seeing one constructive interference determined by the dominant peak.
Looking at this we can see that by breathing we have been able to shift the colour of the film from blue to yellow. The presence of one dominant peak is an indication of the thickness of the film as we are only seeing one constructive interference determined by the dominant peak.
 +
 +
<FONT COLOR="FF0000"> To view the changing film on its own click on this Youtube [http://www.youtube.com/watch?v=VyQ8rDl7qkU link] </FONT>
 +
 +
<FONT COLOR="00FF00"> To view dynamic spectral data click on this Youtube [http://www.youtube.com/watch?v=msEa1X7p8Tg link] </FONT>
 +
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<FONT COLOR="0000FF"> To view full dynamic spectral data with embedded film simultaneously click on this Youtube [http://www.youtube.com/watch?v=aPHo772Wcoo link] </FONT>
==Double Layer Spectra==
==Double Layer Spectra==
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|[[File:cam_Test3.png|thumb|200px|center|Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration]]
|[[File:cam_Test3.png|thumb|200px|center|Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration]]
|}</center>
|}</center>
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This spectra was captured from a double layer with first layer PDMS, 2nd layer reflectin. You can see from the photos that this film is much thicker than before with multiple maxima and minima where the maxima indicate constructive interference and minima indicating destructive interference. The major difference between the two layer and three layer films is primarily there is a greater shift in wavelength because there is no hydrophobic PDMS layer on top preventing swelling of the reflectin layer. We show in the PDMS control that the hydrophobic PDMS does not swell under breathing. Therefore colouration changes in multilayers are all due to reflectin.
 +
 +
<FONT COLOR="FF0000"> To view the changing film on its own click on this Youtube [http://www.youtube.com/watch?v=tL5DlNNZqW8 link] </FONT>
 +
 +
<FONT COLOR="00FF00"> To view dynamic spectral data click on this Youtube [http://www.youtube.com/watch?v=pfWmwR2IrO8 link] </FONT>
 +
 +
<FONT COLOR="0000FF"> To view dynamic spectral data with embedded film changing colour click on this Youtube [http://www.youtube.com/watch?v=PHxhp8Dg5H8 link] </FONT>
==Triple Layer Spectra==
==Triple Layer Spectra==
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|[[File:cam_Test2.png|thumb|200px|center|Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration]]
|[[File:cam_Test2.png|thumb|200px|center|Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration]]
|}</center>
|}</center>
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The three layer spectra incidently should be more distinguishable however as mentioned with the dialyzed proteins they possessed a tendency to dewet which forms a non-uniform layer onto which the PDMS does not very well leading to a very non-uniform third layer which is also very thin.
 +
 +
<FONT COLOR="FF0000"> To view the changing film on its own click on this Youtube [http://www.youtube.com/watch?v=apQXyVEHE3c link] </FONT>
 +
 +
<FONT COLOR="00FF00"> To view dynamic spectral data click on this Youtube [http://www.youtube.com/watch?v=BnZY5k98p-0 link] </FONT>
 +
 +
<FONT COLOR="0000FF"> To view full dynamic spectral data alongside the film click on this Youtube [http://www.youtube.com/watch?v=Ze0BqgfrKxM link] </FONT>
==PDMS Control==
==PDMS Control==
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No response was observed under breathing except a mist of water droplets formed on top which quickly evaporated which incidentally indicates the layer is hydrophobic. This conclusively shows colour changes are due to swelling of the reflectin layers not PDMS.
 +
 +
<FONT COLOR="FF0000">Please see Youtube link </FONT>[http://www.youtube.com/watch?v=RE4N1wodXtU/.avi here]
 +
 +
==Realtime Demo==
 +
 +
I have also uploaded to youtube two video footage taken from my phone of the filming process for the spectral data with full view of the computer screen.
 +
 +
First video footage can be found [http://www.youtube.com/watch?v=pMQb0J-XFmg here].
 +
Second video footage can be found [http://www.youtube.com/watch?v=i_ip8rsdJIs here].
 +
=Acknowledgements=
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We are obliged to Dr Matthew Hawkeye for helping us render the spectral data obtained from the microscope into video and graphs.
{{Template:Team:Cambridge/CAM_2011_EXPERIMENT_FOOT}}
{{Template:Team:Cambridge/CAM_2011_EXPERIMENT_FOOT}}

Latest revision as of 03:53, 22 September 2011

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OVERVIEW
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Contents

Spectral Measurements of Thin Films

With the tris dialyzed samples we recorded spectral data to show that the films being spun were truly 'thin film' and we demonstrated the ability of the thin films to change colour upon breathing by recording video and spectral data when breathed upon under the microscope.

Single Layer Spectra

Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis at the point where breathing was applied
Spectral data slices, they have been displaced in order to better illustrate the changes in profile with time with black indicating before breathing and red after
Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration

Looking at this we can see that by breathing we have been able to shift the colour of the film from blue to yellow. The presence of one dominant peak is an indication of the thickness of the film as we are only seeing one constructive interference determined by the dominant peak.

To view the changing film on its own click on this Youtube [http://www.youtube.com/watch?v=VyQ8rDl7qkU link]

To view dynamic spectral data click on this Youtube [http://www.youtube.com/watch?v=msEa1X7p8Tg link]

To view full dynamic spectral data with embedded film simultaneously click on this Youtube [http://www.youtube.com/watch?v=aPHo772Wcoo link]

Double Layer Spectra

Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis at the point where breathing was applied
Spectral data slices, they have been displaced in order to better illustrate the changes in profile with time with black indicating before breathing and red after
Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration

This spectra was captured from a double layer with first layer PDMS, 2nd layer reflectin. You can see from the photos that this film is much thicker than before with multiple maxima and minima where the maxima indicate constructive interference and minima indicating destructive interference. The major difference between the two layer and three layer films is primarily there is a greater shift in wavelength because there is no hydrophobic PDMS layer on top preventing swelling of the reflectin layer. We show in the PDMS control that the hydrophobic PDMS does not swell under breathing. Therefore colouration changes in multilayers are all due to reflectin.

To view the changing film on its own click on this Youtube [http://www.youtube.com/watch?v=tL5DlNNZqW8 link]

To view dynamic spectral data click on this Youtube [http://www.youtube.com/watch?v=pfWmwR2IrO8 link]

To view dynamic spectral data with embedded film changing colour click on this Youtube [http://www.youtube.com/watch?v=PHxhp8Dg5H8 link]

Triple Layer Spectra

Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis at the point where breathing was applied
Spectral data slices, they have been displaced in order to better illustrate the changes in profile with time with black indicating before breathing and red after
Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration

The three layer spectra incidently should be more distinguishable however as mentioned with the dialyzed proteins they possessed a tendency to dewet which forms a non-uniform layer onto which the PDMS does not very well leading to a very non-uniform third layer which is also very thin.

To view the changing film on its own click on this Youtube [http://www.youtube.com/watch?v=apQXyVEHE3c link]

To view dynamic spectral data click on this Youtube [http://www.youtube.com/watch?v=BnZY5k98p-0 link]

To view full dynamic spectral data alongside the film click on this Youtube [http://www.youtube.com/watch?v=Ze0BqgfrKxM link]

PDMS Control

No response was observed under breathing except a mist of water droplets formed on top which quickly evaporated which incidentally indicates the layer is hydrophobic. This conclusively shows colour changes are due to swelling of the reflectin layers not PDMS.

Please see Youtube link [http://www.youtube.com/watch?v=RE4N1wodXtU/.avi here]

Realtime Demo

I have also uploaded to youtube two video footage taken from my phone of the filming process for the spectral data with full view of the computer screen.

First video footage can be found [http://www.youtube.com/watch?v=pMQb0J-XFmg here].

Second video footage can be found [http://www.youtube.com/watch?v=i_ip8rsdJIs here].

Acknowledgements

We are obliged to Dr Matthew Hawkeye for helping us render the spectral data obtained from the microscope into video and graphs. Back to Experiments