Team:Cambridge/Experiments/Reflectin Thin Films VII

From 2011.igem.org

(Difference between revisions)
(Triple Layer Spectra)
(Triple Layer Spectra)
Line 33: Line 33:
The three layer spectra incidently should be more distinguishable however as mentioned with the dialyzed proteins they possessed a tendency to dewet which forms a non-uniform layer onto which the PDMS does not very well leading to a very non-uniform third layer which is also very thin.
The three layer spectra incidently should be more distinguishable however as mentioned with the dialyzed proteins they possessed a tendency to dewet which forms a non-uniform layer onto which the PDMS does not very well leading to a very non-uniform third layer which is also very thin.
-
<FONT COLOR="FF0000"> To view the changing film on its own click on this Youtube [[http://www.youtube.com/watch?v=apQXyVEHE3c link]] </FONT>
+
<FONT COLOR="FF0000"> To view the changing film on its own click on this Youtube [http://www.youtube.com/watch?v=apQXyVEHE3c link] </FONT>
-
<FONT COLOR="00FF00"> To view dynamic spectral data click on this Youtube [[http://www.youtube.com/watch?v=apQXyVEHE3c link]] </FONT>
+
<FONT COLOR="00FF00"> To view dynamic spectral data click on this Youtube [http://www.youtube.com/watch?v=apQXyVEHE3c link] </FONT>
==PDMS Control==
==PDMS Control==

Revision as of 00:02, 22 September 2011

Loading...
OVERVIEW
home
Back to Experiments

Contents

Spectral Measurements of Thin Films

With the tris dialyzed samples we recorded spectral data to show that the films being spun were truly 'thin film' and we demonstrated the ability of the thin films to change colour upon breathing by recording video and spectral data when breathed upon under the microscope.

Single Layer Spectra

Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis at the point where breathing was applied
Spectral data slices, they have been displaced in order to better illustrate the changes in profile with time with black indicating before breathing and red after
Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration

Looking at this we can see that by breathing we have been able to shift the colour of the film from blue to yellow. The presence of one dominant peak is an indication of the thickness of the film as we are only seeing one constructive interference determined by the dominant peak.

Double Layer Spectra

Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis at the point where breathing was applied
Spectral data slices, they have been displaced in order to better illustrate the changes in profile with time with black indicating before breathing and red after
Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration

This spectra was captured from a double layer with first layer PDMS, 2nd layer reflectin. You can see from the photos that this film is much thicker than before with multiple maxima and minima where the maxima indicate constructive interference and minima indicating destructive interference. The major difference between the two layer and three layer films is primarily there is a greater shift in wavelength because there is no hydrophobic PDMS layer on top preventing swelling of the reflectin layer. We show in the PDMS control that the hydrophobic PDMS does not swell under breathing. Therefore colouration changes in multilayers are all due to reflectin.

Triple Layer Spectra

Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis at the point where breathing was applied
Spectral data slices, they have been displaced in order to better illustrate the changes in profile with time with black indicating before breathing and red after
Contour plot of reflectance (height) against wavelength along the x-axis and time along the y-axis for the entire capture duration showing the film reverts back to its original colouration

The three layer spectra incidently should be more distinguishable however as mentioned with the dialyzed proteins they possessed a tendency to dewet which forms a non-uniform layer onto which the PDMS does not very well leading to a very non-uniform third layer which is also very thin.

To view the changing film on its own click on this Youtube [http://www.youtube.com/watch?v=apQXyVEHE3c link]

To view dynamic spectral data click on this Youtube [http://www.youtube.com/watch?v=apQXyVEHE3c link]

PDMS Control

No response was observed under breathing except a mist of water droplets formed on top which quickly evaporated which incidentally indicates the layer is hydrophobic.

Please see Youtube link [http://www.youtube.com/watch?v=RE4N1wodXtU/.avi here]


Back to Experiments