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Talk:Team:UEA-JIC Norwich/Weekfive
From 2011.igem.org
Monday 11th July 2011
A start to the week with everyone contemplating what has already been done and what we think is best to move forward successfully with the project, unbelievable how fast week 5 came along. We also performed a transformation in E.coli using the G-Luc sent to them from a Cambridge research group.
Tuesday 12th July 2011
Our E.coli cells with the G-Luc plasmid grew nicely and we then picked off a colony to make a culture which was left to grow overnight.
Wednesday 13th July 2011
Today a miniprep was performed on our transformed E.coli cells containing the G-Luc plasmid, it looks like everything went according to plan. A glycerol stock of the transformed cells was also made so we can use them for future experiments.
Thursday 14th July 2011
The Moss people of the team decided to start pouring plates today for a near future transformation. A gel of our miniprep from the previous day was performed today, sadly there was no presence of a band for the G-Luc. Today the team did not have much luck.
Friday 15th July 2011
Another attempt miniprepping the transformed E.coli cells with G-Luc was performed today, all hoping tomorrow we see a band present in the gel.
