User contributions

From 2011.igem.org

(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)

• 05:28, 29 September 2011 (diff | hist) N File:Picture-9.jpg ‎ (top)
• 05:25, 29 September 2011 (diff | hist) N File:Picture-8.jpg ‎ (top)
• 05:23, 29 September 2011 (diff | hist) N File:Picture-7.jpg ‎ (top)
• 05:22, 29 September 2011 (diff | hist) N File:Picture-6.jpg ‎ (top)
• 05:21, 29 September 2011 (diff | hist) N File:Picture-5.jpg ‎ (top)
• 05:20, 29 September 2011 (diff | hist) N File:Picture-4.jpg ‎ (top)
• 05:19, 29 September 2011 (diff | hist) N File:Picture-3.jpg ‎ (top)
• 05:17, 29 September 2011 (diff | hist) N File:Picture-2.jpg ‎ (top)
• 05:16, 29 September 2011 (diff | hist) N File:Picture-1.jpg ‎ (top)
• 04:27, 29 September 2011 (diff | hist) Team:Panama/16 June 2011 ‎ (top)
• 04:25, 29 September 2011 (diff | hist) Team:Panama/8 June 2011 ‎ (top)
• 04:24, 29 September 2011 (diff | hist) Team:Panama/7 June 2011 ‎ (top)
• 04:18, 29 September 2011 (diff | hist) Team:Panama/22 September 2011 ‎ (top)
• 04:14, 29 September 2011 (diff | hist) N Team:Panama/23 September 2011 ‎ (Created page with "==23 Septembre 2011== '''We did:''' 1. Straight forward muthageneris. 2. PCR again to complete chain. 3. Electrophoresis of it. 4. Second attempt of SFM PCR") (top)
• 04:11, 29 September 2011 (diff | hist) N Team:Panama/20 September 2011 ‎ (Created page with " ==20 Septembre 2011== '''We did:''' 1. Transformation again with K-12 and JM109 competent cells beacuse last transformation didn't results.") (top)
• 04:06, 29 September 2011 (diff | hist) N Team:Panama/26 August 2011 ‎ (Created page with " ==26 August 2011== '''We did:''' 1. Prepare aliquots of glicerol 40% of GFP + Terminator. 2. Miniprep of re-culture of GFP + Terminator") (top)
• 04:04, 29 September 2011 (diff | hist) N Team:Panama/25 August 2011 ‎ (Created page with " ==25 August 2011== '''We did:''' 1. Enzymatic digestion of GFP + Terminator. 2. Electrophoresis of miniprep.") (top)
• 04:03, 29 September 2011 (diff | hist) Team:Panama/24 August 2011 ‎ (→Auguts 24, 2011) (top)
• 04:02, 29 September 2011 (diff | hist) N Team:Panama/24 August 2011 ‎ (Created page with " ==Auguts 24, 2011== '''We did:''' 1. Prepare aliquots of 50 microM of primers. 2. Revision of gel of electrophoresis of PCR 8/22/11 for have more DNA concentrated for mutagen...")
• 03:59, 29 September 2011 (diff | hist) N Team:Panama/22 August 2011 ‎ (Created page with " ==Auguts 22, 2011== '''We did:''' 1. Eelectrophoresis of PCR of RhlAB. 2. Transformation. 3. Purification of RhlAB PCR, Measure with nanodrop (Results 35 ng/ml :(! ) 4. New...") (top)
• 03:57, 29 September 2011 (diff | hist) N Team:Panama/19 August 2011 ‎ (Created page with " ==Auguts 19, 2011== '''We did:''' -Verify the growing of Expression Plasmid.") (top)
• 03:56, 29 September 2011 (diff | hist) N Team:Panama/18 August 2011 ‎ (Created page with " ==Auguts 18, 2011== '''We did:''' 1. Prepare glicerol stocks of induced Expression Plasmid and miniprep of it. 2. Broth of Biobricks with salt and hydrocarbons tolerance and...") (top)
• 03:52, 29 September 2011 (diff | hist) N Team:Panama/17 August 2011 ‎ (Created page with " ==August 17, 2011== '''We did:''' 1. Revise the plates with transformation of the new competent cells. 2. Biobrick transformation of TUDelft. (Alkane hidroxyase, Salt toleran...") (top)
• 03:46, 29 September 2011 (diff | hist) N Team:Panama/16 August 2011 ‎ (Created page with " ==August 16, 2011== '''We did:''' 1. DNA purification. 2. Transformation of competent cells.") (top)
• 03:45, 29 September 2011 (diff | hist) Team:Panama/15 August 2011 ‎ (→August 15, 2011) (top)
• 03:43, 29 September 2011 (diff | hist) N Team:Panama/15 August 2011 ‎ (Created page with " ==August 15, 2011== '''We did:''' 1-Preparation of TAE 1X. 2. Electrophoresis in TAE 1X with products of PCR with Rh1AB primers (8/14/11). 3.Autoclave LB medium for competen...")
• 03:33, 29 September 2011 (diff | hist) N File:IGEM means 3.JPG ‎ (top)
• 03:32, 29 September 2011 (diff | hist) N File:IGEM measn 2.JPG ‎ (top)
• 03:31, 29 September 2011 (diff | hist) N File:IGEM means1.jpg ‎ (top)
• 03:06, 29 September 2011 (diff | hist) N Team:Panama/21 July 2011 ‎ (Created page with "==July 21, 2011== '''We did:''' 1. Prepared agar LB (500 ml) and ampiciline (50 microliters/ml). 2. Digestions with Pst1 of our Rh1AB gen purified with PCR. 3. Check operatio...") (top)
• 02:59, 29 September 2011 (diff | hist) N Team:Panama/19 July 2011 ‎ (Created page with "==July 19, 2011== '''We did:''' 1. Digestion using Pst1 on Biobrick, purified Rh1AB mutated and Rh1AB gen.") (top)
• 02:57, 29 September 2011 (diff | hist) N Team:Panama/18 July 2011 ‎ (Created page with "==July 18, 2011== '''We did:''' Interpretation of PCR of 7/14/2011. Chronogram of activities of the week.") (top)
• 02:55, 29 September 2011 (diff | hist) N Team:Panama/14 July 2011 ‎ (Created page with "=='''July 14, 2011'''== '''We did:''' 1. Use nanodrop to measure concentrations of yesterday miniprep. We obtained questionable results because concentrationsdo not follow the e...") (top)
• 02:39, 29 September 2011 (diff | hist) Team:Panama/13 July 2011 ‎ (→July 13, 2011) (top)
• 02:37, 29 September 2011 (diff | hist) Team:Panama/13 July 2011 ‎ (→July 13, 2011)
• 02:29, 29 September 2011 (diff | hist) N Team:Panama/13 July 2011 ‎ (Created page with "=='''July 13, 2011'''== '''We did:''' 1. Electrophoresis of the products of yesterday PCR and 3 digestions. '''What we expect to know?''' If EcoR1 enzyme digest and in how muc...")
• 02:21, 29 September 2011 (diff | hist) N Team:Panama/12 July 2011 ‎ (Created page with "=='''July 12, 2011'''== '''We did:''' 1. Digestion 2. PCR of pure positives control of miniprep (A1), negative control and dilutions of the gen 2010. Dilution of positive contr...") (top)
• 02:15, 29 September 2011 (diff | hist) N Team:Panama/11 July 2011 ‎ (Created page with "=='''July 11, 2011'''== '''We did:''' 1. Dilutions of the gen Rh1ab (2010) and of the samples of last miniprep in TE buffer. 2. PCR with internal primers. 3. Electrophoresis i...") (top)
• 02:11, 29 September 2011 (diff | hist) Team:Panama/8 July 2011 ‎ (→July 8, 2011) (top)
• 02:11, 29 September 2011 (diff | hist) N Team:Panama/8 July 2011 ‎ (Created page with "=='''July 8, 2011'''== '''We did:''' Enzymatic digestion of the gen with la Xba1 enzyme during 2 hours at 37ºC.")
• 02:06, 29 September 2011 (diff | hist) N Team:Panama/6 July 2011 ‎ (Created page with "=='''July 6, 2011'''== '''We did:''' 1. PCR of the miniprep of Biobrick gen and electrophoresis of the digestion product. 2. PCR of: -gen Rh1AB, (0,5 microliters)(1) -gen Rh1...") (top)
• 01:41, 29 September 2011 (diff | hist) N Team:Panama/5 July 2011 ‎ (Created page with "=='''July 5, 2011'''== '''We did:''' 1. Enzimatic digestion using digestion protocol . We made digestion of the 3 parts of the platform of expression (promotor + Rbs, RhlAB) and...") (top)
• 01:31, 29 September 2011 (diff | hist) N Team:Panama/1 July 2011 ‎ (Created page with "=='''July 1, 2011'''== We did: 1. A PCR of the last miniprep to determine if what we extract was right. We use the PCR protocol for Rht gen [https://2011.igem.org/Team:Panama/P...") (top)
• 00:58, 29 September 2011 (diff | hist) N Team:Panama/30 June 2011 ‎ (Created page with "=='''June 30, 2011'''== '''We did:''' 1. Miniprep of the parts P+RBS and GFP+T.") (top)
• 00:51, 29 September 2011 (diff | hist) Team:Panama/29 June 2011 ‎ (→June 29, 2011) (top)
• 00:46, 29 September 2011 (diff | hist) Team:Panama/TheTeam ‎ (→Students)
• 00:37, 29 September 2011 (diff | hist) Team:Panama/TheTeam ‎ (→Sergio Mosquera)
• 00:08, 29 September 2011 (diff | hist) Team:Panama/TheTeam ‎ (→Paul Monterrey)
• 23:58, 28 September 2011 (diff | hist) Team:Panama/TheTeam ‎
• 02:07, 13 July 2011 (diff | hist) Team:Panama/TheTeam ‎ (→Who we are)

(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)