"

From 2011.igem.org

July 12, 2011

We did:

1. Digestion

2. PCR of pure positives control of miniprep (A1), negative control and dilutions of the gen 2010. Dilution of positive control mentioned, negative control and absolute gen Rh1ab in 45 microliters of TE buffer with serial dilutions. Were used external primers.

3. Prepared chloramphenicol, then pass colonies in LB medium with this and incubate it at 37ºC.