Team:Tokyo Metropolitan/Notebook
From 2011.igem.org
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=Lab Protocols= | =Lab Protocols= | ||
==PCR== | ==PCR== | ||
+ | *Ice | ||
+ | *Micro pipette | ||
+ | *Thermal cycler | ||
+ | *PCR Tube | ||
+ | *Micro Tube | ||
+ | *DW | ||
+ | *5× PCR Buffer | ||
+ | *dNTP (2.5mM) | ||
+ | *Primer(Forward & Reverse) (20µM) | ||
+ | *Template DNA | ||
+ | *PrimeSTAR® HS DNA Polymerase (2.5U/μl) | ||
+ | |||
==Electrophoresis== | ==Electrophoresis== | ||
==Ethanol precipitation== | ==Ethanol precipitation== |
Revision as of 08:23, 4 October 2011
Contents |
Lab Notebook
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Lab Protocols
PCR
- Ice
- Micro pipette
- Thermal cycler
- PCR Tube
- Micro Tube
- DW
- 5× PCR Buffer
- dNTP (2.5mM)
- Primer(Forward & Reverse) (20µM)
- Template DNA
- PrimeSTAR® HS DNA Polymerase (2.5U/μl)