Team:Tokyo Metropolitan/Notebook

From 2011.igem.org

(Difference between revisions)
(Lab Protocols)
(PCR)
Line 206: Line 206:
=Lab Protocols=
=Lab Protocols=
==PCR==
==PCR==
 +
*Ice
 +
*Micro pipette
 +
*Thermal cycler
 +
*PCR Tube
 +
*Micro Tube
 +
*DW
 +
*5× PCR Buffer
 +
*dNTP (2.5mM)
 +
*Primer(Forward & Reverse)  (20µM)
 +
*Template DNA
 +
*PrimeSTAR® HS DNA Polymerase (2.5U/μl)
 +
==Electrophoresis==
==Electrophoresis==
==Ethanol precipitation==
==Ethanol precipitation==

Revision as of 08:23, 4 October 2011

Contents

Lab Notebook

Augast
Sun Mon Tue Wed Thu Fri Sat
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31
September
Sun Mon Tue Wed Thu Fri Sat
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30
Octorber
Sun Mon Tue Wed Thu Fri Sat
1
2 3 4 5 6


Lab Protocols

PCR

  • Ice
  • Micro pipette
  • Thermal cycler
  • PCR Tube
  • Micro Tube
  • DW
  • 5× PCR Buffer
  • dNTP (2.5mM)
  • Primer(Forward & Reverse)  (20µM)
  • Template DNA
  • PrimeSTAR® HS DNA Polymerase (2.5U/μl)

Electrophoresis

Ethanol precipitation

Plasmid Extraction

Digestion

Ligation

Transformation

Colony PCR

Making Medium for Culture

Making Gel for Electrophoresis