-Visualization-

-Plasmid extraction to DNA refining for pSB1C3+oriTr-GFP no.5.

Used QIAGEN's kits. Master plate of colony PCR showed flourescent red, thus ligation might be failed.

-Mesurement of DNA concentration.

pSB1C3+oriTr-GFP no.5 178.5 ng/ul

-Colony PCR

Samples which transformed one day ago

-Speeding up-

-Purify products of PCR

-Ligation and Transform BBa_K543001