-Visualization-

-oriTr-GFP＋oriTr Electophoresis to check for colony PCR

-oriTr-GFP＋oriTr Plasmid extraction to DNA refining

-oriTr-GFP＋oriTr Digestion

-oriTr-GFP＋oriTr Electrophoresis

Results

Ligation might be failed. Results from sequence, some samples lost oriTr.

-Colony PCR

Checked for results promoter - suppressed expression system (Cm)