-Speeding up-

Ligation HNS(wt) and BBa_I20260

Transformation to JM109

-Visualization-

Ethanol precipitation of J23100,I714051.

Concentration of J23100,I714051 was still low.

@transformation OriTr+GFP and OriT, but failure

-Targeting-

-Plasmid extraction

Used QlaprepSpinMiniprepkit

Results

J23100

Jaiko 123

Jian 82

P0440

Pisuke 158

Pichiku 155

(ng/ul)

-Electrophoresis

Checked for K317037 and J23100

Results

K317037 and J23100 had cut

-Gel extraction

Gel extracted to DNA refined

Results for concentration

Kenedy 20

Keit 32

Jason 0

Jiro 10

-Digestion

J23100: S and P

P0440: X adn P

-Preculture

Precultured K317037, then incubated at 37 deg C.

Made 50*TAEBuffer