-Visualization-

@confirmation of transformation　(yesterday)

@restriction enzyme to pSB1C3(backbone for parts) (EcoRI, SpeⅠ)

@electrophore pSB1C3, O+G, total 4 samples.

@restriction enzyme to pSB1C3 (EcoRI, SpeⅠ)

@ligation OG+O ①

@transformation OG+O ①

@ligation OG+O ②

@transformation OG+O ②

-Plasmid extraction

Promoter and suppressed expression system

-DNA refining

Above samples

-Digestion

Above samples

-Electrophoresis

Above samples

-Digestion

Promoter: Spe and Pst

Suppressed expression system: Xba and Pst

-Targetting-

@electrophoresis J23100-K317037, K283030, J22005
J23100-K317037, K283030 find binds.
J22005 not find bind.

@gel extraction and confirmation binds by electrophoresis J23100-K317037, K283030

@ligation J23100-K317037 and K283030

@transformation J23100-K317037-K283030