-Targeting-

-Plasmid extraction and DNA refining

Extracted plasmids for targetingDevice and P0440.

Results

Targeting

75 ng/ul

P0440

20 ng/ul

-Electrophoresis

Checked for P0440, Targeting and P0440.

Results

Checked to cut for P0440 by treat restriction enzyme but length was 1000 bp (too long).

-Transformation

Transformed P0140 instead of P0440. After, incubated at 37 deg C.

-Visualization-

-Colony PCR

Samples which transformed one day ago

-Electrophoresis

Above samples