Team:Queens Canada/tour2

From 2011.igem.org

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<h3green> Our Summer Project</h3green><p>
<h3green> Our Summer Project</h3green><p>
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<regulartext> This summer we engineered the nematode worm <i> C. elegans </i> to build a bioremediation toolkit.  </regulartext> <p>
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<regulartext> To get a quick overview of our project, take a look of the video to the left where Adrian, Stephanie, Tony and Allister give a rundown of the summer objectives. <p></regulartext>  
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<h3green> Transgenic Chemotaxis  </h3green> <p>
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<regulartext> The principal goal of our project, modifying the worm's normal chemotaxis mechanism to increase it's affinity for toxic chemicals, was accomplished by creating genetic constructs that were then injected into <i> C. elegans </i> worms.  </regulartext> <p>
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<regulartext> A breakdown of the findings can be found on the <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Project/Results">results</a><span> page, while the video gallery offers a glimpse of our worms in action! </regulartext>
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<img align="left" style="margin-bottom:0px; width:356px; vertical-align: middle; padding:0;" src="https://static.igem.org/mediawiki/2011/1/1f/Queens_Canada_let858ox1runc54.png">
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<h3green> </h3green><p>
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<h3green> QGEM: Queen's iGEM Team <p></h3green><p>
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<h3green> Reporter System  <p></h3green><p>
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<regulartext> The International Genetically Engineered Machine (iGEM) team at Queen's University is called QGEM, composed of a group of undergraduate students from Kingston, Canada. </regulartext> <p> <regulartext> We spent the summer in the lab working on the nematode worm <i> C. elegans </i> to create a bioremediation toolkit.</regulartext> <p>
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<regulartext> When targeting a pollutant, we researched an effective method to assess the concentration of chemical present. Our findings are outline <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Side/Reporter">here</a><span>.  </regulartext> <p>
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<regulartext> As a first step, we successfully engineered a worm to express a <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Parts/Featured">cyan fluorescent protein</a><span> in a chemotaxis neuron! </regulartext>
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<regulartext> Meet <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Intro/Team">the team </a><span>,  inspiring <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Intro/Team">Faculty Advisors</a><span>, or check out <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Intro/Photos">photos </a><span>from the summer! </regulartext> <p>
 
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<h3green> Methods & Results </h3green>
 
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<regulartext> background info </regulartext>
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<h3green> </h3green><p>
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<h3green> Biodegradation Mechanism <p></h3green><p>
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<regulartext> A key component of the bioremediation toolkit is a <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Side/Biodegradation">biodegradation mechanism</a><span>. We examined the pathway for breakdown of naphthalene and incorporated this into our design.  </regulartext> <p>
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<regulartext> Find out more about a featured <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Parts/Featured">biobrick coding for the nahD enzyme</a><span>.
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<h3green> </h3green><p>
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<h3green> Use Our Parts  <p></h3green><p>
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<regulartext>  All of <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Parts/Registry">our parts</a><span>  are available to be used in your future synthetic biology project. We submitted 10 modular and 16 composite constructs to the <span class="classgreent"><a href="http://partsregistry.org/">iGEM parts registry</a><span>. For future iGEM teams, we also included a few intermediate constructs to build from. </regulartext> <p>
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<regulartext> Take a look at our  <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Parts/Featured">featured parts </a><span>of 2011 and <span class="classgreent"><a href="https://2011.igem.org/Team:Queens_Canada/Parts/Contributions">novel assembly method</a><span> for synthetic biologists. </regulartext>
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<b>
<span class="classgreen"><a href="https://2011.igem.org/Team:Queens_Canada/tour1"> << back </a><span>
<span class="classgreen"><a href="https://2011.igem.org/Team:Queens_Canada/tour1"> << back </a><span>
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<span class="classgreen"><a href="https://2011.igem.org/Team:Queens_Canada/tour3">continue to human practices >></a><span>
<span class="classgreen"><a href="https://2011.igem.org/Team:Queens_Canada/tour3">continue to human practices >></a><span>
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Latest revision as of 04:03, 29 October 2011

Queen's
Our Summer Project

This summer we engineered the nematode worm C. elegans to build a bioremediation toolkit.

To get a quick overview of our project, take a look of the video to the left where Adrian, Stephanie, Tony and Allister give a rundown of the summer objectives.

Transgenic Chemotaxis

The principal goal of our project, modifying the worm's normal chemotaxis mechanism to increase it's affinity for toxic chemicals, was accomplished by creating genetic constructs that were then injected into C. elegans worms.

A breakdown of the findings can be found on the results page, while the video gallery offers a glimpse of our worms in action!

Reporter System

When targeting a pollutant, we researched an effective method to assess the concentration of chemical present. Our findings are outline here.

As a first step, we successfully engineered a worm to express a cyan fluorescent protein in a chemotaxis neuron!

Biodegradation Mechanism

A key component of the bioremediation toolkit is a biodegradation mechanism. We examined the pathway for breakdown of naphthalene and incorporated this into our design.

Find out more about a featured biobrick coding for the nahD enzyme.

Use Our Parts

All of our parts are available to be used in your future synthetic biology project. We submitted 10 modular and 16 composite constructs to the iGEM parts registry. For future iGEM teams, we also included a few intermediate constructs to build from.

Take a look at our featured parts of 2011 and novel assembly method for synthetic biologists.

Retrieved from "http://2011.igem.org/Team:Queens_Canada/tour2"