Team:Queens Canada/Safety/FAQs

Would the materials used in your project and/or your final product pose:

a. Risks to the safety and health of team members or others in the lab?
The potential carcinogen ethidium bromide was used in our gel electrophoresis experiments. We followed our labs standard protocols for dealing with the substance, which includes the use of protective material and steps to minimize the use of ethidium bromide. As well, the carcinogen naphthalene was used in our chemotaxis assays. Care was taken to handle all chemicals in a well-ventilated area, using appropriate safety attire. b. Risks to the safety and health of the general public if released by design or accident?
In any transgenic organism, a potential risk could be posed to human and ecosystem health due to their novel genetics conferring an evolutionary advantage over wild type organisms. Given this, this project is designed to minimize the possibility that such an event could occur. Caenorhabditis elegans is not known to be pathogenic in humans. Furthermore, the extrachromosomal assay used in the microinjection process greatly weakens the constitution of transgenetic C.elegans. Therefore, if it did escape in the wild, it would be even less likely to be pathogenic to humans than wild type, and would probably be outcompeted by wild type C.elegans in all environments. a. Risks to the safety and health of team members or others in the lab?
The potential carcinogen ethidium bromide was used in our gel electrophoresis experiments. We followed our labs standard protocols for dealing with the substance, which includes the use of protective material and steps to minimize the use of ethidium bromide. As well, the carcinogen naphthalene was used in our chemotaxis assays. Care was taken to handle all chemicals in a well-ventilated area, using appropriate safety attire. a. Risks to the safety and health of team members or others in the lab?
The potential carcinogen ethidium bromide was used in our gel electrophoresis experiments. We followed our labs standard protocols for dealing with the substance, which includes the use of protective material and steps to minimize the use of ethidium bromide. As well, the carcinogen naphthalene was used in our chemotaxis assays. Care was taken to handle all chemicals in a well-ventilated area, using appropriate safety attire.

Would the materials used in your project and/or your final product pose:

a. Risks to the safety and health of team members or others in the lab?
The potential carcinogen ethidium bromide was used in our gel electrophoresis experiments. We followed our labs standard protocols for dealing with the substance, which includes the use of protective material and steps to minimize the use of ethidium bromide. As well, the carcinogen naphthalene was used in our chemotaxis assays. Care was taken to handle all chemicals in a well-ventilated area, using appropriate safety attire. a. Risks to the safety and health of team members or others in the lab?
The potential carcinogen ethidium bromide was used in our gel electrophoresis experiments. We followed our labs standard protocols for dealing with the substance, which includes the use of protective material and steps to minimize the use of ethidium bromide. As well, the carcinogen naphthalene was used in our chemotaxis assays. Care was taken to handle all chemicals in a well-ventilated area, using appropriate safety attire. a. Risks to the safety and health of team members or others in the lab?
The potential carcinogen ethidium bromide was used in our gel electrophoresis experiments. We followed our labs standard protocols for dealing with the substance, which includes the use of protective material and steps to minimize the use of ethidium bromide. As well, the carcinogen naphthalene was used in our chemotaxis assays. Care was taken to handle all chemicals in a well-ventilated area, using appropriate safety attire. a. Risks to the safety and health of team members or others in the lab?
The potential carcinogen ethidium bromide was used in our gel electrophoresis experiments. We followed our labs standard protocols for dealing with the substance, which includes the use of protective material and steps to minimize the use of ethidium bromide. As well, the carcinogen naphthalene was used in our chemotaxis assays. Care was taken to handle all chemicals in a well-ventilated area, using appropriate safety attire.