Team:Cambridge/Labwork/Protocols

From 2011.igem.org

(Difference between revisions)
(Protocols)
(Protocols)
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*[[Team:Cambridge/Protocols/Spin Coating |Spin Coating to Make a Thin Film]]: Our Spin Coating Protocol.
*[[Team:Cambridge/Protocols/Spin Coating |Spin Coating to Make a Thin Film]]: Our Spin Coating Protocol.
*[[Team:Cambridge/Protocols/Flow_coating | Flow Coating]]:  How to flow coat a thin film.
*[[Team:Cambridge/Protocols/Flow_coating | Flow Coating]]:  How to flow coat a thin film.
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*[[Team:Cambridge/Protocols/Flow_coating | Altering Substrate Surface Chemistry]]:  Various methods to alter the surface chemistry of silicon and PDMS to achieve better wetting and thin film production
'''Gel Electrophoresis by SDS PAGE'''
'''Gel Electrophoresis by SDS PAGE'''

Revision as of 22:47, 20 September 2011

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OVERVIEW
home

Protocols

A list of all protocols developed during the project. We used this page as a reference throughout the competition.

Amplification of DNA

  • Polymerase Chain Reaction : A method for amplifying a section of DNA.
  • Colony PCR : PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.

Analysis of DNA

Preparation of DNA Constructs

  • Primer Design : Some general guidelines on how to design successful primers.
  • Gibson Assembly : An extremely powerful technique for joining multiple, arbitrary DNA sequences in one step, compatible with standard assembly.

Transformation of Bacterial Cells

Bacterial Cultures

Extraction of DNA

Microscopy

Protein Purification

Thin Film Preparation

Gel Electrophoresis by SDS PAGE