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Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
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*[https://2008.igem.org/Team:Hawaii/Protocols/Competent_Cells TOP10 Competency Protocol] | *[https://2008.igem.org/Team:Hawaii/Protocols/Competent_Cells TOP10 Competency Protocol] | ||
*[[Team:Brown-Stanford/Lab/Protocols/FastCaCl|Fast CaCl<sub>2</sub> Combined Competency and Transformation]] | *[[Team:Brown-Stanford/Lab/Protocols/FastCaCl|Fast CaCl<sub>2</sub> Combined Competency and Transformation]] | ||
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| + | === '''Assembly''' === | ||
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| + | *[[Team:Brown-Stanford/Lab/Protocols/Gibson|Gibson Assembly]] | ||
=== '''Equipment''' === | === '''Equipment''' === | ||
Revision as of 21:17, 30 June 2011
-
REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
-
Lab
-
Partners
-
Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
Protocols
Media
- BG-11
- LB (Lysogeny broth)
- ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- Enrichment media
- Nutrient agar with urea
- SOB (Super Optimal Broth) and SOC (Super Optimal broth with Catabolite repression)
Competency and Transformations
- Registry Distribution Transformation
- TOP10 Competency Protocol
- Fast CaCl2 Combined Competency and Transformation
Assembly
Equipment
