Team:Amsterdam/9 May 2011
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{{:Team:Amsterdam/Header}} | {{:Team:Amsterdam/Header}} | ||
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= '''Meeting 9''' = | = '''Meeting 9''' = | ||
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'''Funding''' | '''Funding''' | ||
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*Some rejections were made | *Some rejections were made | ||
=='''Projects discussion'''== | =='''Projects discussion'''== | ||
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'''Methane producing ''E.coli''''' | '''Methane producing ''E.coli''''' | ||
*Sequences of the genes were found | *Sequences of the genes were found | ||
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*One protein is membrane bound, this is a problem, because these proteins fold different in different membranes of organisms. | *One protein is membrane bound, this is a problem, because these proteins fold different in different membranes of organisms. | ||
*How to check the methane production? | *How to check the methane production? | ||
- | + | *Try to produce '''ethylene''' instead of methane | |
'''Ethylene producing ''E.coli''''' | '''Ethylene producing ''E.coli''''' | ||
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*It can be edited to be more heat resistent | *It can be edited to be more heat resistent | ||
*Applications: | *Applications: | ||
- | ** | + | **Attracting bacteria (extra receptor) |
**Use it in plants | **Use it in plants | ||
***A lot of responses in plants to ethylene | ***A lot of responses in plants to ethylene | ||
- | ==='''Neglected | + | ==='''Neglected diseases'''=== |
'''Kala-azar''' | '''Kala-azar''' | ||
*Parasite called ''Leishmania'' | *Parasite called ''Leishmania'' |
Revision as of 12:30, 6 July 2011
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Meeting 9
Funding
- NISB sponsored us!
- Some rejections were made
Projects discussion
Methane producing E.coli
- Sequences of the genes were found
- 3 multi subunit proteins
- One protein is membrane bound, this is a problem, because these proteins fold different in different membranes of organisms.
- How to check the methane production?
- Try to produce ethylene instead of methane
Ethylene producing E.coli
- Only one enzyme is needed and a product in TCA cycle
- 3 2-oxoglutarate ⇒ 2 ethylene + succinate
- Plasmid is already available
- It can be edited to be more heat resistent
- Applications:
- Attracting bacteria (extra receptor)
- Use it in plants
- A lot of responses in plants to ethylene
Neglected diseases
Kala-azar
- Parasite called Leishmania
- Cell necrosis in skin
- Present diagnostic is with antibodies
- Peanut agglutin is possible binding target
- Search for membrane proteins as targets
Hookworm
- It secretes a PAMP
- Find a receptor that binds the PAMP
Other possible diseases
- Chagas disease
What should be checked for each disease
- Expense
- Diagnose time present
- Diagnose time with new bac
- How quick is the disease infection/critical point
- Incubation time
- PAMP
- Receptor
- Life stage detection and life expectancy
- Sample it can be detected in
Opportunistic infections
- Leishmania spp
- Toxoplasma spp.
- Pneumocystis jiroveci