Team:Amsterdam/10 September 2011
From 2011.igem.org
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10-09-2011
Constructs
A colony PCR was performed on the transformants from 09-09-2011. A total of 19 colonies were selected. The colony PCR was checked on agarose gel. No sample showed the expected band sizes.
Backbone transferring
Ligation of different parts with the Chloramphenicol backbone:
- Cpn10
- Cpn 60
- CspC
- K538101
- K538104
The ligation was performed ON at 16°C.