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Team:Amsterdam/8 September 2011
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(Created page with "=08-09-2011= ==Constructs== The following parts were miniprepped: *Cpn10 *Cpn60 *CspC *K538100 *K538101 *K538104 *K538120 *K538130 *K538200 *Shednak *R0010 *K206000 ===Digestio...") |
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*The constructs we've miniprepped today | *The constructs we've miniprepped today | ||
These parts were ligated into the Chloramphenicol backbone ON at 16°C. | These parts were ligated into the Chloramphenicol backbone ON at 16°C. | ||
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Revision as of 08:19, 21 September 2011
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08-09-2011
Constructs
The following parts were miniprepped:
- Cpn10
- Cpn60
- CspC
- K538100
- K538101
- K538104
- K538120
- K538130
- K538200
- Shednak
- R0010
- K206000
Digestion
Constructs making using the 2a assembly method. Digestion of different parts to construct the following assemblies:
- R0010+ B0034 + CspC
- R0010+ B0034 + Cpn60
- R0010+ B0034 + Shednak
- K206000+ B0034 + CspC
- K206000+ B0034 + Cpn60
- K206000+ B0034 + Shednak
These assemblies were ligated ON at 16°C.
Backbone transferring
Digestion of different parts to tranfer to the Chloramphenicol backbone:
- All genes
- The constructs we've miniprepped today
These parts were ligated into the Chloramphenicol backbone ON at 16°C.
