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Team:Amsterdam/14 September 2011
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==Growth curves== | ==Growth curves== | ||
| - | Measuring growth curves off our first construct!! We measure Cpn10 with a lacz | + | Measuring growth curves off our first construct!! We measure Cpn10 with a lacz promoter in a ampicillin backbone. As control we take Top 10 cells with an ampicillin resistance. Today we measured at 4, 8, 15 and 21°C. <br> |
| + | |||
| + | ==Construct checks== | ||
| + | The following constructs, that showed a good result with colony PCR, were miniprepped: | ||
| + | *Cpn60 in pSB1C3 | ||
| + | *K538101 in pSB1C3 | ||
| + | *K538104 in pSB1C3 | ||
| + | Afterwards they were digested and checked on gel. | ||
| + | All showed a good result on gel. | ||
| + | |||
| + | ==Transformation== | ||
| + | The ON ligation samples were transformed into a ''E. coli'' TOP 10 strain. | ||
{{:Team:Amsterdam/Footer}} | {{:Team:Amsterdam/Footer}} | ||
Latest revision as of 10:34, 21 September 2011
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14-09-2011
Growth curves
Measuring growth curves off our first construct!! We measure Cpn10 with a lacz promoter in a ampicillin backbone. As control we take Top 10 cells with an ampicillin resistance. Today we measured at 4, 8, 15 and 21°C.
Construct checks
The following constructs, that showed a good result with colony PCR, were miniprepped:
- Cpn60 in pSB1C3
- K538101 in pSB1C3
- K538104 in pSB1C3
Afterwards they were digested and checked on gel. All showed a good result on gel.
Transformation
The ON ligation samples were transformed into a E. coli TOP 10 strain.
