13-09-2011
Growth curves
Measuring growth curves off our first construct!! We measure Cpn10 with a lacz promoter in a ampicillin backbone. As control we take Top 10 cells with an ampicillin resistance. Today we start the measurements at 4 and 8°C. (Tomorow 4, 8, 15 and 21°C will be tested)
Construct checks
The following constructs, that showed a good result with colony PCR, were miniprepped:
- Cpn60 in pSB1C3
- K538101 in pSB1C3
- K538104 in pSB1C3
- Cpn10 in pSB1C3
Afterwards they were digested and checked on gel.
Only Cpn10 showed a good result, the rest showed no bands on gel.
Those were checked again on the 14-09-2011.
Constructs
All digestions from 12-09-2011 were purified using an agarose gel and a gel extraction kit.
The purified samples were checked on an agarose gel. All samples showed a good result, except K538101.
Those samples were used for an ON ligation at 16°C.