Team:Amsterdam/13 September 2011
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==Growth curves== | ==Growth curves== | ||
Measuring growth curves off our first construct!! We measure Cpn10 with a lacz promoter in a ampicillin backbone. As control we take Top 10 cells with an ampicillin resistance. Today we start the measurements at 4 and 8°C. (Tomorow 4, 8, 15 and 21°C will be tested)<br> | Measuring growth curves off our first construct!! We measure Cpn10 with a lacz promoter in a ampicillin backbone. As control we take Top 10 cells with an ampicillin resistance. Today we start the measurements at 4 and 8°C. (Tomorow 4, 8, 15 and 21°C will be tested)<br> | ||
+ | |||
+ | ==Construct checks== | ||
+ | The following constructs, that showed a good result with colony PCR, were miniprepped: | ||
+ | *Cpn60 in Psb1C3 | ||
+ | *K538101 in Psb1C3 | ||
+ | *K538104 in Psb1C3 | ||
+ | *Cpn10 in Psb1C3 | ||
+ | Afterwards they were digested and checked on gel. | ||
+ | Only Cpn10 showed a good result, the rest showed no bands on gel. | ||
+ | Those were checked again on the 14-09-2011. | ||
+ | |||
+ | ==Constructs== | ||
+ | All digestions from 12-09-2011 were purified using an agarose gel and a gel extraction kit.<br> | ||
+ | The purified samples were checked on an agarose gel. All samples showed a good result, except K538101. | ||
+ | Those samples were used for an ON ligation at 16°C. | ||
{{:Team:Amsterdam/Footer}} | {{:Team:Amsterdam/Footer}} |
Revision as of 09:46, 21 September 2011
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13-09-2011
Growth curves
Measuring growth curves off our first construct!! We measure Cpn10 with a lacz promoter in a ampicillin backbone. As control we take Top 10 cells with an ampicillin resistance. Today we start the measurements at 4 and 8°C. (Tomorow 4, 8, 15 and 21°C will be tested)
Construct checks
The following constructs, that showed a good result with colony PCR, were miniprepped:
- Cpn60 in Psb1C3
- K538101 in Psb1C3
- K538104 in Psb1C3
- Cpn10 in Psb1C3
Afterwards they were digested and checked on gel. Only Cpn10 showed a good result, the rest showed no bands on gel. Those were checked again on the 14-09-2011.
Constructs
All digestions from 12-09-2011 were purified using an agarose gel and a gel extraction kit.
The purified samples were checked on an agarose gel. All samples showed a good result, except K538101.
Those samples were used for an ON ligation at 16°C.