Team:Amsterdam/11 May 2011
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==Ethylene producing ''E.coli''== | ==Ethylene producing ''E.coli''== | ||
*The project is to short | *The project is to short |
Revision as of 13:21, 6 July 2011
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11-05-2011 | VU Meeting for topics discussion
Ethylene producing E.coli
- The project is to short
- Are there alternative ways to meassure ethylene?
- Use colors to check if ethylene is produced
- Ethylene sensing is difficult, because of the receptor.
- Evolution doesn't create biobricks
Neglected disease
Kala-azar
- Is the protein available?
- Whole parasite is a problem
- Signal transduction via peanut agglutin can't be done
- Check if peanut agglutin binds to other stuff in human body fluids
- We need specialists on this subject
- Look at Imperial college, they've done a project on parasites
- Target was an excreted protein
Hookworm
- CCL11 will be cut and luciferase fades when the hookworm is in the sample
- Can't we do this the other way around
- Metalloproteases cleaves the CCl11, if this isn't available it doesn't promote luciferase production
- Can this be done in E.coli
- Are CCL11 and metalloproteases available for in the lab?
Buruli ulcer
- It is a bacteria
- It is checked with FRET
- Can the specific receptor be palced in E.coli
- The pathogen is known, but no detection method yet
- Can mycolactone be detected?
- Membrane receptor can be a problem
- Maybe use Mycobacteria