Team:Amsterdam/11 May 2011
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**Target was an excreted protein | **Target was an excreted protein | ||
- | ='''Hookworm'''= | + | ===='''Hookworm'''==== |
*CCL11 will be cut and luciferase fades when the hookworm is in the sample | *CCL11 will be cut and luciferase fades when the hookworm is in the sample | ||
**Can't we do this the other way around | **Can't we do this the other way around | ||
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*Are CCL11 and metalloproteases available for in the lab? | *Are CCL11 and metalloproteases available for in the lab? | ||
- | ='''Buruli ulcer'''= | + | ===='''Buruli ulcer'''==== |
*It is a bacteria | *It is a bacteria | ||
*It is checked with FRET | *It is checked with FRET |
Revision as of 12:33, 6 July 2011
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VU Meeting for topics discussion
Ethylene producing E.coli
- The project is to short
- Are there alternative ways to meassure ethylene?
- Use colors to check if ethylene is produced
- Ethylene sensing is difficult, because of the receptor.
- Evolution doesn't create biobricks
Neglected disease
Kala-azar
- Is the protein available?
- Whole parasite is a problem
- Signal transduction via peanut agglutin can't be done
- Check if peanut agglutin binds to other stuff in human body fluids
- We need specialists on this subject
- Look at Imperial college, they've done a project on parasites
- Target was an excreted protein
Hookworm
- CCL11 will be cut and luciferase fades when the hookworm is in the sample
- Can't we do this the other way around
- Metalloproteases cleaves the CCl11, if this isn't available it doesn't promote luciferase production
- Can this be done in E.coli
- Are CCL11 and metalloproteases available for in the lab?
Buruli ulcer
- It is a bacteria
- It is checked with FRET
- Can the specific receptor be palced in E.coli
- The pathogen is known, but no detection method yet
- Can mycolactone be detected?
- Membrane receptor can be a problem
- Maybe use Mycobacteria