Team:Amsterdam/11 May 2011
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{{:Team:Amsterdam/Header}} | {{:Team:Amsterdam/Header}} | ||
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= '''VU Meeting for topics discussion''' = | = '''VU Meeting for topics discussion''' = | ||
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'''Ethylene producing ''E.coli''''' | '''Ethylene producing ''E.coli''''' | ||
*The project is to short | *The project is to short | ||
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*Whole parasite is a problem | *Whole parasite is a problem | ||
*Signal transduction via peanut agglutin can't be done | *Signal transduction via peanut agglutin can't be done | ||
- | ** | + | **Check if peanut agglutin binds to other stuff in human body fluids |
*We need specialists on this subject | *We need specialists on this subject | ||
*Look at Imperial college, they've done a project on parasites | *Look at Imperial college, they've done a project on parasites | ||
**Target was an excreted protein | **Target was an excreted protein | ||
- | '''Hookworm''' | + | ='''Hookworm'''= |
*CCL11 will be cut and luciferase fades when the hookworm is in the sample | *CCL11 will be cut and luciferase fades when the hookworm is in the sample | ||
**Can't we do this the other way around | **Can't we do this the other way around | ||
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*Are CCL11 and metalloproteases available for in the lab? | *Are CCL11 and metalloproteases available for in the lab? | ||
- | '''Buruli ulcer''' | + | ='''Buruli ulcer'''= |
*It is a bacteria | *It is a bacteria | ||
*It is checked with FRET | *It is checked with FRET | ||
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*Membrane receptor can be a problem | *Membrane receptor can be a problem | ||
*Maybe use Mycobacteria | *Maybe use Mycobacteria | ||
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{{:Team:Amsterdam/Footer}} | {{:Team:Amsterdam/Footer}} |
Revision as of 12:32, 6 July 2011
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VU Meeting for topics discussion
Ethylene producing E.coli
- The project is to short
- Are there alternative ways to meassure ethylene?
- Use colors to check if ethylene is produced
- Ethylene sensing is difficult, because of the receptor.
- Evolution doesn't create biobricks
Neglected disease
Kala-azar
- Is the protein available?
- Whole parasite is a problem
- Signal transduction via peanut agglutin can't be done
- Check if peanut agglutin binds to other stuff in human body fluids
- We need specialists on this subject
- Look at Imperial college, they've done a project on parasites
- Target was an excreted protein
Hookworm
- CCL11 will be cut and luciferase fades when the hookworm is in the sample
- Can't we do this the other way around
- Metalloproteases cleaves the CCl11, if this isn't available it doesn't promote luciferase production
- Can this be done in E.coli
- Are CCL11 and metalloproteases available for in the lab?
Buruli ulcer
- It is a bacteria
- It is checked with FRET
- Can the specific receptor be palced in E.coli
- The pathogen is known, but no detection method yet
- Is mycolactone detactable?
- Membrane receptor can be a problem
- Maybe use Mycobacteria