Team:Amsterdam/11 May 2011
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{{:Team:Amsterdam/Header}} | {{:Team:Amsterdam/Header}} | ||
- | = | + | {{:Team:Amsterdam/Notebook/Calendar}} |
- | + | =11-05-2011 | VU Meeting for topics discussion= | |
- | + | ==Ethylene producing ''E.coli''== | |
- | + | ||
*The project is to short | *The project is to short | ||
*Are there alternative ways to meassure ethylene? | *Are there alternative ways to meassure ethylene? | ||
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*Evolution doesn't create biobricks | *Evolution doesn't create biobricks | ||
- | === | + | ===Neglected disease=== |
- | + | ====Kala-azar==== | |
*Is the protein available? | *Is the protein available? | ||
*Whole parasite is a problem | *Whole parasite is a problem | ||
*Signal transduction via peanut agglutin can't be done | *Signal transduction via peanut agglutin can't be done | ||
- | ** | + | **Check if peanut agglutin binds to other stuff in human body fluids |
*We need specialists on this subject | *We need specialists on this subject | ||
*Look at Imperial college, they've done a project on parasites | *Look at Imperial college, they've done a project on parasites | ||
**Target was an excreted protein | **Target was an excreted protein | ||
- | + | ====Hookworm==== | |
*CCL11 will be cut and luciferase fades when the hookworm is in the sample | *CCL11 will be cut and luciferase fades when the hookworm is in the sample | ||
**Can't we do this the other way around | **Can't we do this the other way around | ||
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*Are CCL11 and metalloproteases available for in the lab? | *Are CCL11 and metalloproteases available for in the lab? | ||
- | + | ====Buruli ulcer==== | |
*It is a bacteria | *It is a bacteria | ||
*It is checked with FRET | *It is checked with FRET | ||
*Can the specific receptor be palced in ''E.coli'' | *Can the specific receptor be palced in ''E.coli'' | ||
*The pathogen is known, but no detection method yet | *The pathogen is known, but no detection method yet | ||
- | * | + | *Can mycolactone be detected? |
*Membrane receptor can be a problem | *Membrane receptor can be a problem | ||
*Maybe use Mycobacteria | *Maybe use Mycobacteria | ||
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{{:Team:Amsterdam/Footer}} | {{:Team:Amsterdam/Footer}} |
Latest revision as of 10:28, 21 September 2011
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11-05-2011 | VU Meeting for topics discussion
Ethylene producing E.coli
- The project is to short
- Are there alternative ways to meassure ethylene?
- Use colors to check if ethylene is produced
- Ethylene sensing is difficult, because of the receptor.
- Evolution doesn't create biobricks
Neglected disease
Kala-azar
- Is the protein available?
- Whole parasite is a problem
- Signal transduction via peanut agglutin can't be done
- Check if peanut agglutin binds to other stuff in human body fluids
- We need specialists on this subject
- Look at Imperial college, they've done a project on parasites
- Target was an excreted protein
Hookworm
- CCL11 will be cut and luciferase fades when the hookworm is in the sample
- Can't we do this the other way around
- Metalloproteases cleaves the CCl11, if this isn't available it doesn't promote luciferase production
- Can this be done in E.coli
- Are CCL11 and metalloproteases available for in the lab?
Buruli ulcer
- It is a bacteria
- It is checked with FRET
- Can the specific receptor be palced in E.coli
- The pathogen is known, but no detection method yet
- Can mycolactone be detected?
- Membrane receptor can be a problem
- Maybe use Mycobacteria