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Team:UEA-JIC Norwich/electrophoresis
From 2011.igem.org
UNIVERSITY OF EAST ANGLIA-JOHN INNES CENTRE
1. Measure 0.6g of agarose and add to 60 mL TAE Buffer
2. Mix throroughly
3. Heat until solution becomes clear
4. Allow to cool, then add 30µl of 1mg per ml Ethidium Bromide
5. Pour solution into gel tray with comb in place
6. Allow to set
7. Place in electrophoresis tank and submerge in TAE buffer
8. Pipette 10 µl of ladder into left most well
9. Perform serial dilution of sample
10. Add 9 µl of sample to 1 µl of loading dye and place in wells, noting position of each sample
11. Run gel at 90V for 30 minutes
2. Visualise using UV light
