Team:Cambridge/Protocols/Gel Electrophoresis of Protein

Protein Analysis by SDS PAGE

These are some notes made in preparation for running SDS PAGE to verify we have Reflectin.

A 12% PAGE gel will separate 12kDa-60kDa proteins

Theory

How it works

Practice

How to do it in the lab

{Standard layout for procedures is to use:

• <Procedure title - aka what you are doing>

1. <step 1>
2. <step 2>
   • <additional notes/important information regarding the previous step>

the text within the < > is what should be written, don't include < > in actual writeup :P

if in doubt see the gel electrophoresis protocol

}

Safety

The safety implication of the procedure.