Team:Cambridge/Protocols/Preparing Slides With Agarose Supplement To Form Microcolonies

Preparing Slides With Agarose Supplement To Form Microcolonies

This protocol was adapted from this video protocol: [http://www.jove.com/details.php?id=3145 |Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy Imke G. de Jong et al. Molecular Genetics Group, University of Groningen]

Theory

How it works

Practice

How to do it in the lab

{Standard layout for procedures is to use:

• <Procedure title - aka what you are doing>

1. <step 1>
2. <step 2>
   • <additional notes/important information regarding the previous step>

the text within the < > is what should be written, don't include < > in actual writeup :P

if in doubt see the gel electrophoresis protocol

}

Safety

The safety implication of the procedure.