Team:Cambridge/Experiments/Isolating Reflectin DNA

From 2011.igem.org

Revision as of 11:17, 20 July 2011 by Cat (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Loading...

iGEM HQ

Page Source

Intro Source

OVERVIEW

home

You do not appear to have JavaScript enabled.Without it, you will be able to view the rest of the website, but you will not be able to view the helpful introductions in this panel.

Obtaining DNA coding for reflectin proteins was the vital first step in our project. [http://en.wikipedia.org/wiki/Loligo Loligo] tissue was sourced from fishing bait suppliers and culinary wholesalers in order to attempt genomic DNA extraction. From our literature search we discovered that the reflectin gene from E. scolopes contains no introns, so genomic DNA should be suitable for expression in E. coli.

Squid Dissection and Tissue Samples

Specimens of what we identified as [http://www.marlin.ac.uk/speciesinformation.php?speciesID=3718 Loligo vulgaris] and [http://en.wikipedia.org/wiki/Opalescent_Inshore_Squid Loligo opalescens] were dissected for examination.