Team:Cambridge/Protocols/Restriction Enzyme Digestion

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Restriction Enzyme Digestion

protocol description

Theory

How it works

Practice

Master mix preparation

2.0μl of DNA

0.5μl of each restriction enzyme (or a respective amount of water for the control with uncut plasmids)

0.1μl of BSA - acetylated Bovine Serum Albumin enhances the performance of restriction enzymes

5.9μl of water

1.0μl of 2×NEBuffer

Safety

The safety implication of the procedure.