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Team:Cambridge/Protocols/Gel Electrophoresis
From 2011.igem.org
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Gel Electrophoresis
A method to separate DNA strands of different lengths.
Theory
DNA is negatively charged due to the phosphate ions in its backbone. Hence, when an electric field is applied across an electrolyte such as agarose gel the DNA fragments tend to move towards the cathode. The smaller the fragment, the less resistance it experiences from the surrounding gel, and hence the further it moves in a given time.
Practice
How to do it in the lab
Safety
The safety implication of the procedure.
