Team:Cambridge/Experiments/Initial Exercise Group A

Initial Exercise: Cat, Jonathan, Haydn and Ai

In order to get to grips with the techniques we'll need for this summer, we were tasked with creating an interesting GFP fusion

Primer Design

The plasmid vector we were supplied with contains a strong promoter upstream of a sfGFP coding sequence. Our fusion design relies on amplifying the ftsZ coding region from Bacillus and creating regions of overlap between this and the GFP coding sequence in the plasmid, in order to create the gene fusion.