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Team:Cambridge/Parts
From 2011.igem.org
System diagrams
Our parts were designed for the two main branches of our labwork - in vivo expression and export to try and achieve structural colour, and overexpression for purification and in vitro studies of our recombinant reflectins.
Overexpression for purification
Export
Featured Parts
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638001 Reflectin A1]
Reflectins are a family of proteins which help give cephalopods their amazing camouflage abilities and which can self-assemble into a variety of multilayered structures with optical properties. We worked with Reflectin A1 from Loligo pealeii. See our background page for more information about reflectins and their role in cephalopod camouflage.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638202 Reflectin A1 with poly-His tag]
In pure form reflectins may be used to create vibrantly coloured thin films and other devices. We used a poly-His affinity column to purify reflectin from cells for this purpose.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638402 Improved TorA tag]
We submitted a variant of the TorA leader sequence export tag. This TorA leader sequence variant has had been successfully used to export GFP to the periplasm of E.coli as described [http://www.ncbi.nlm.nih.gov/pubmed/11123687 here]. Our tag differs slightly from [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233307 the TorA tag already in the registry] and should be cheaper to obtain from primer synthesis.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638401 Reflectin A1 with N-terminal TorA tag]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638403 Reflectin A1 with N-terminal TorA tag and C-terminal sfGFP]
...and the rest
A complete list of parts submitted to the Registry by this year's Cambridge team can be found below. (We also added our [http://partsregistry.org/Part:BBa_I0500:Experience experience of part I0500] to the registry.) <groupparts>iGEM011 Cambridge</groupparts>
