Team:Cambridge/Protocols/Glycerol Stocks

Glycerol Stocks

protocol description

1. Peparation of liqid cultures - 100-fold dilution of overnight culture with selection. 2. Incubation at 37° for 3-4 hours until mid-log phase. 3. Mix with 60% glycerol to get a final concentration of 20%. 4. Store at -80°

Theory

How it works

Practice

How to do it in the lab

{Standard layout for procedures is to use:

• <Procedure title - aka what you are doing>

1. <step 1>
2. <step 2>
   • <additional notes/important information regarding the previous step>

the text within the < > is what should be written, don't include < > in actual writeup :P

if in doubt see the gel electrophoresis protocol

}

Safety

The safety implication of the procedure.