Periplasmic export

As reflectins appear to spontaneously associate with membranes, we hoped to promote correct assembly by directing our recombinant reflectins to the [http://en.wikipedia.org/wiki/Periplasmic_space periplasmic space of E. coli].

Design of constructs

Choice of signal sequences

The signal sequence from TorA [http://www.ncbi.nlm.nih.gov/pubmed/11123687 has been used to export GFP to the periplasm]. Although

GFP imaging

Conclusion