Team:Cambridge/Experiments/Assembly of Reflectin Constructs

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iGEM HQ

Page Source

Intro Source

OVERVIEW

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Construct Design

Primer Design

Assembly: first attempt

PCR

In the first round of PCR, we amplified fragments required for the assembly of GA1, GA2, GA3 and GA4 constructs.

Team:Cambridge/Experiments/Assembly of Reflectin Constructs

From 2011.igem.org

Contents

Construct Design

Primer Design

Assembly: first attempt

PCR

Gibson Assembly

Transformation

Results

Diagnostics

Assembly: second attempt

PCR

Gibson Assembly

Transformation

Results

What next?